Antimicrobial susceptibility and multilocus sequence typing of Mycoplasma capricolum subsp. capricolum

• Published in: PloS one Vol. 12; no. 3; p. e0174700
• Main Authors: Tatay-Dualde, Juan, Prats-van der Ham, Miranda, de la Fe, Christian, Paterna, Ana, Sánchez, Antonio, Corrales, Juan Carlos, Contreras, Antonio, Tola, Sebastiana, Gómez-Martin, Ángel
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 27.03.2017; Public Library of Science (PLoS)
• Subjects: Agalactia; Animal health; Anti-Infective Agents - pharmacology; Antibacterial agents; Antiinfectives and antibacterials; Antimicrobial agents; Antimicrobial resistance; Bacteria; Bacterial Proteins - genetics; Biological evolution; Biology and Life Sciences; Browning; Cattle; Causes of; Chemical industry; Ciprofloxacin; Clindamycin; Clusters; Colony-forming cells; Computer and Information Sciences; Contagious bovine pleuropneumonia; Data processing; Deoxyribonucleic acid; Diagnosis; Dilution; Disease susceptibility; DNA; DNA topoisomerase; Dosage and administration; Doxycycline; Drug resistance; Enrofloxacin; Epidemiology; Erythromycin; Evolutionary genetics; Fluoroquinolones; Format; Fragmentation; Fragments; Genes; Genetic aspects; Genetic diversity; Genetic variability; Genetic Variation; Genetics; Guidelines; Infections; Infectious diseases; Iron; Islands; Italy; Macrolides - pharmacology; Medicine and Health Sciences; Minimum inhibitory concentration; Molecular biology; Multilocus Sequence Typing; Mycoplasma; Mycoplasma capricolum; Mycoplasma capricolum - drug effects; Mycoplasma capricolum - genetics; Mycoplasma capricolum capricolum; Nucleic acids; Outbreaks; Pathogenesis; pH effects; Pharmacokinetics; Pharmacology; Phylogeny; Plates; Pleuropneumonia; Polymerase chain reaction; Quantitation; Spain; Studies; Tetracyclines; United States > US; Spain
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0174700

Mycoplasma capricolum subsp. capricolum is one of the causative agents of contagious agalactia (CA). Nevertheless, there is still a lack of information about its antimicrobial susceptibility and genetic characteristics. Therefore, the aim of this work was to study the antimicrobial and genetic variability of different Mycoplasma capricolum subsp. capricolum field isolates. For this purpose, the growth inhibition effect of 18 antimicrobials and a multilocus sequence typing (MLST) scheme based on five housekeeping genes (fusA, glpQ, gyrB, lepA and rpoB) were performed on 32 selected field isolates from Italy and Spain.The results showed a wide range of growth inhibitory effects for almost all the antimicrobials studied. Macrolides presented lower efficacy inhibiting Mcc growth than in previous works performed on other CA-causative mycoplasmas. Erythromycin was not able to inhibit the growth of any of the studied strains, contrary to doxycycline, which inhibited the growth of all of them from low concentrations. On the other hand, the study of the concatenated genes revealed a high genetic variability among the different Mcc isolates. Hence, these genetic variations were greater than the ones reported in prior works on other mycoplasma species.