McWRI1, a transcription factor of the AP2/SHEN family, regulates the biosynthesis of the cuticular waxes on the apple fruit surface under low temperature

• Published in: PloS one Vol. 12; no. 10; p. e0186996
• Main Authors: Hao, Suxiao, Ma, Yiyi, Zhao, Shuang, Ji, Qianlong, Zhang, Kezhong, Yang, Mingfeng, Yao, Yuncong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.10.2017; Public Library of Science (PLoS)
• Subjects: Agricultural products; Agriculture; Alcohol; Alkanes; Apples; Biology and Life Sciences; Biosynthesis; Cloning, Molecular; Cuticular wax; Deoxyribonucleic acid; DNA; DNA, Complementary - genetics; Enzymes; Fatty acids; Freshness; Fruit - genetics; Fruit - metabolism; Fruits; Gene expression; Gene Expression Regulation, Plant; Gene Silencing; Genes; Genetic aspects; Genomics; Kinases; Laboratories; Low temperature; Malus - genetics; Malus - metabolism; Malus - physiology; Malus asiatica; Malus sieversii; Metabolism; Morphology; Mutation; Physical Sciences; Physiological aspects; Plant protection; Plant Proteins - genetics; Plant Proteins - metabolism; Plant sciences; Promoter Regions, Genetic - genetics; Research and Analysis Methods; Seeds; Shelf life; Storage; Stress, Physiological - genetics; Temperature; Temperature effects; Transcription factors; Transcription Factors - deficiency; Transcription Factors - genetics; Transcription Factors - metabolism; Waxes - metabolism; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0186996

Cuticular waxes of plant and organ surfaces play an important role in protecting plants from biotic and abiotic stress and extending the freshness, storage time and shelf life in the post-harvest agricultural products. WRI1, a transcription factor of AP2/SHEN families, had been found to trigger the related genes taking part in the biosynthesis of seed oil in many plants. But whether WRI1 is involved in the biosynthesis of the cuticular waxes on the Malus fruits surface has been unclear. We investigated the changes of wax composition and structure, the related genes and WRI1 expression on Malus asiatica Nakai and sieversii fruits with the low temperature treatments, found that low temperature induced the up-regulated expression of McWRI1, which promoted gene expression of McKCS, McLACs and McWAX in very-long-chain fatty acid biosynthesis pathway, resulting in the accumulation of alkanes component and alteration of wax structure on the fruit surface. Corresponding results were verified in McWRI1 silenced by VIGS, and WRI1 silenced down-regulated the related genes on two kinds of fruits, it caused the diversity alteration in content of some alkanes, fatty acid and ester component in two kinds of fruits. We further conducted Y1H assay to find that McWRI1 transcription factor activated the promoter of McKCS, McLAC and McWAX to regulate their expression. These results demonstrated that McWRI1 is involved in regulating the genes related synthesis of very long chain fatty acid on surface of apple fruits in storage process, providing a highlight for improvement of the modified atmosphere storage of apple fruits.