Determination of factors associated with natural soil suppressivity to potato common scab

• Published in: PloS one Vol. 10; no. 1; p. e0116291
• Main Authors: Sagova-Mareckova, Marketa, Daniel, Ondrej, Omelka, Marek, Kristufek, Vaclav, Divis, Jiri, Kopecky, Jan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.01.2015; Public Library of Science (PLoS)
• Subjects: Agricultural practices; Bacteria; Calcium; Chromatography; Communities; Community structure; Crop diseases; Crop rotation; Cultivars; Disease control; Ecology; Epidemiology; Experiments; Genes, Bacterial; High performance liquid chromatography; Highlands; Indoles - metabolism; Iron; Liquid chromatography; Magnesium; Microorganisms; Multigene Family; Nitrogen; Organic matter; Pathogenicity; Pathogens; pH effects; Piperazines - metabolism; Plant communities; Plant diseases; Plant Diseases - microbiology; Plant Tubers - microbiology; Plants (botany); Polymorphism; Polymorphism, Restriction Fragment Length; Potato common scab; Potatoes; Seeds; Soil acidity; Soil analysis; Soil bacteria; Soil chemistry; Soil Microbiology; Soil microorganisms; Soil pH; Soils; Solanum tuberosum - microbiology; Streptomyces; Streptomyces - genetics; Streptomyces - metabolism; Streptomyces - pathogenicity; Sulfur; TxtB gene
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0116291

Common scab of potatoes is a disease, which is difficult to manage due to complex interactions of the pathogenic bacteria (Streptomyces spp.) with soil, microbial community and potato plants. In Bohemian-Moravian Highlands in the Czech Republic two sites (Vyklantice and Zdirec) were selected for a study of common scab disease suppressivity. At both sites, a field with low disease severity occurs next to one with high severity and the situation was regularly observed over four decades although all four fields undergo a crop rotation. In the four fields, quantities of bacteria, actinobacteria and the gene txtB from the biosynthetic gene cluster of thaxtomin, the main pathogenicity factor of common scab, were analyzed by real-time PCR. Microbial community structure was compared by terminal fragment length polymorphism analysis. Soil and potato periderm were characterized by contents of carbon, nitrogen, phosphorus, sulphur, calcium, magnesium, and iron. Quality of organic matter was assessed by high performance liquid chromatography of soil extracts. The study demonstrated that the suppressive character of the fields is locally specific. At Zdirec, the suppressivity was associated with low txtB gene copies in bulk soil, while at Vyklantice site it was associated with low txtB gene copies in the tuberosphere. The differences were discussed with respect to the effect of abiotic conditions at Zdirec and interaction between potato plant and soil microbial community at Vyklantice. Soil pH, Ca soil content or cation concentrations, although different were not in the range to predict the disease severity. Low severity of common scab was associated with low content of soil C, N, C/N, Ca and Fe suggesting that oligotrophic conditions may be favorable to common scab suppression.