The Effectiveness of Crataegus orientalis M Bieber. (Hawthorn) Extract Administration in Preventing Alveolar Bone Loss in Rats with Experimental Periodontitis

• Published in: PloS one Vol. 10; no. 6; p. e0128134
• Main Authors: Hatipoğlu, Mükerrem, Sağlam, Mehmet, Köseoğlu, Serhat, Köksal, Ekrem, Keleş, Ali, Esen, Hacı Hasan
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.06.2015; Public Library of Science (PLoS)
• Subjects: Alveolar bone; Alveolar Bone Loss - complications; Alveolar Bone Loss - prevention & control; Animals; Antioxidants; Antioxidants (Nutrients); Antioxidants - metabolism; Biocompatibility; Biomedical materials; Bone loss; Bone marrow; Computed tomography; Crataegus - chemistry; Dentistry; Disease; Disease Models, Animal; Drug dosages; Ethanol; Flavonoids; Fruits; Gum disease; Inflammation; Laboratory animals; Male; Mandible; Medical research; Molars; Osteoblasts; Osteoclasts; Oxidants - blood; Oxidative stress; Oxidative Stress - drug effects; Periodontal disease; Periodontitis; Periodontitis - blood; Periodontitis - complications; Periodontitis - diagnostic imaging; Periodontitis - metabolism; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Rats; Rats, Wistar; Rodents; Silk; Studies; Submerging; Teeth; Tumor necrosis factor-TNF; X-Ray Microtomography; Turkey
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0128134

The purpose of this animal study was to evaluate the effects of hawthorn (Crataeus orientalis M Bieber.) extract on serum oxidative status and alveolar bone loss in experimental periodontitis. Twenty-seven Wistar rats were assigned to one of the following groups: non- ligated+placebo (saline) (NL, n = 9), ligature only+placebo (saline) (LO, n = 9), and ligature and treated with hawthorn extract in saline (H, n = 9) (100 mg/kg orogastrically, once a day for 11 days). Periodontitis was induced by submerging a 4/0 silk ligature in the sulcus of the mandibular right first molars of rats, and the animals were sacrificed after 11 days. Micro-CT examinations were performed for linear and volumetric parameter assessment of alveolar bone. Periodontal tissues were histopathologically examined to assess the differences among the study groups. Levels of serum total antioxidant status (TAS)/total oxidant status (TOS), and oxidative stress index (OSI) were also analyzed. Alveolar bone loss was significantly reduced by hawthorn administration compared to LO group (p<0.05). The number of inflammatory cells and osteoclasts in the LO group was significantly higher than that of the NL and H groups (p< 0.05). The number of osteoblasts in the LO and H groups was significantly higher than that of the NL group (p<0.05). TOS and OSI levels were significantly reduced in H group compared to LO group (P <0.05) and TAS levels were similar in H and NL group (p< 0.05). Hawthorn extract showed inhibitory effect on periodontal inflammation and alveolar bone loss by regulating TAS, TOS and OSI levels in periodontal disease in rats when administered systemically.