Exposure to Hyperbaric Oxygen Intensified Vancomycin-Induced Nephrotoxicity in Rats

• Published in: PloS one Vol. 11; no. 4; p. e0152554
• Main Authors: Sabler, Itay M., Berkovitch, Matitiahu, Sandbank, Judith, Kozer, Eran, Dagan, Zahi, Goldman, Michael, Bahat, Hilla, Stav, Kobi, Zisman, Amnon, Klin, Baruch, Abu-Kishk, Ibrahim
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.04.2016; Public Library of Science (PLoS)
• Subjects: Anesthesia; Animal models; Animals; Antibiotics; Biology and Life Sciences; Blood; Blood levels; Blood Urea Nitrogen; Carbon dioxide; Care and treatment; Creatinine; Creatinine - blood; Disease Models, Animal; Dosage and administration; Drug dosages; Enzymes; Experiments; Exposure; Gene expression; Hyperbaric oxygen therapy; Hyperbaric oxygenation; Hyperbaric Oxygenation - adverse effects; Hyperbaric Oxygenation - methods; Hypoxia; Kidney - drug effects; Kidney diseases; Kidney Diseases - blood; Kidney Diseases - chemically induced; Kidney Function Tests - methods; Kidneys; Magnesium; Magnesium - blood; Male; Medicine; Medicine and Health Sciences; Methods; Nephrology; Oxidative stress; Oxidative Stress - drug effects; Oxygen; Oxygen - adverse effects; Oxygen therapy; Pediatrics; Pharmacology; Physical Sciences; Rats; Rats, Sprague-Dawley; Rodents; Studies; Therapy; Toxicity; Tuberculosis; Urea; Urology; Vancomycin; Vancomycin - adverse effects
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0152554

It has been suggested that oxidative stress is a potential mechanism for vancomycin-induced nephrotoxicity and hyperbaric oxygen therapy (HBO) has been shown to be effective in treating renal toxicity that has been pharmacologically induced in animal models. The aim of this study was to investigate the effect of HBO therapy on vancomycin-induced nephrotoxicity in rats. The study group comprised 36 Sprague Dawley male rats. We treated 30 with 500 mg/kg of intraperitoneal vancomycin once a day for 7 days. Half of these rats received a daily 1-hour treatment with HBO at 2 Atmospheres (ATM) on the same 7 days and formed the HBO+ group. The other 15 subjects received no HBO treatment (HBO- group). The remaining six rats served as the control group, three received HBO treatments alone and no treatment was administered to the other three rats. Laboratory results were obtained on day 8 and the intervention and control groups were compared. Rats in the HBO+ group gained less weight than the HBO- group (11.6 grams vs 22.6 grams; P = 0,008) and had significantly higher serum blood urea nitrogen (99.6 vs 52.6 mg/dL; P<0.001), serum creatinine (0.42 vs 0.16 mg/dL; P = 0.001) and magnesium (3.6 vs 3.1 mg/dL; P = 0.014). The vancomycin blood levels were also higher in the HBO+ group (27.8 vs 6.7 μg/mL; P = 0.078). There were no pathological kidney changes in the control group. All the kidneys from the treated groups (vancomycin +HBO and vancomycin HBO-) showed moderate to severe histopathological changes with no statistical significance between them. This study demonstrated that exposure to hyperbaric oxygen intensified vancomycin-induced nephrotoxicity in rats.