LysM receptors in Coffea arabica: Identification, characterization, and gene expression in response to Hemileia vastatrix

• Published in: PloS one Vol. 17; no. 2; p. e0258838
• Main Authors: Santos, Mariana de Lima, de Resende, Mário Lúcio Vilela, Dos Santos Ciscon, Bárbara Alves, Freitas, Natália Chagas, Pereira, Matheus Henrique de Brito, Reichel, Tharyn, Mathioni, Sandra Marisa
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.02.2022; Public Library of Science (PLoS)
• Subjects: Amino Acid Sequence; Analysis; Arabidopsis - genetics; Arabidopsis Proteins - genetics; Basidiomycota - genetics; Basidiomycota - physiology; Biology and Life Sciences; Chitin; Coffea - genetics; Coffea - metabolism; Coffea - microbiology; Coffea arabica; Coffee; Computer and Information Sciences; Conservation; Conserved sequence; Cultivars; Defense programs; Disease resistance; Engineering and Technology; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Gene Expression; Genetic aspects; Genome, Plant; Genomes; Genomics; Hemileia vastatrix; Homology; Inoculation; Kinases; Nucleotide sequence; Oryza - genetics; Pathogens; Pattern recognition; Pattern recognition receptors; Phylogenetics; Phylogeny; Physical Sciences; Plant breeding; Plant diseases; Plant Diseases - microbiology; Plant Proteins - classification; Plant Proteins - genetics; Plant Proteins - metabolism; Plant species; Proteins; Receptors; Receptors, Pattern Recognition - classification; Receptors, Pattern Recognition - genetics; Receptors, Pattern Recognition - metabolism; Research and Analysis Methods; Sequence Alignment; Similarity; Structure-function relationships; Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0258838

Pathogen-associated molecular patterns (PAMPs) are recognized by pattern recognition receptors (PRRs) localized on the host plasma membrane. These receptors activate a broad-spectrum and durable defense, which are desired characteristics for disease resistance in plant breeding programs. In this study, candidate sequences for PRRs with lysin motifs (LysM) were investigated in the Coffea arabica genome. For this, approaches based on the principle of sequence similarity, conservation of motifs and domains, phylogenetic analysis, and modulation of gene expression in response to Hemileia vastatrix were used. The candidate sequences for PRRs in C. arabica (Ca1-LYP, Ca2-LYP, Ca1-CERK1, Ca2-CERK1, Ca-LYK4, Ca1-LYK5 and Ca2-LYK5) showed high similarity with the reference PRRs used: Os-CEBiP, At-CERK1, At-LYK4 and At-LYK5. Moreover, the ectodomains of these sequences showed high identity or similarity with the reference sequences, indicating structural and functional conservation. The studied sequences are also phylogenetically related to the reference PRRs described in Arabidopsis, rice, and other plant species. All candidates for receptors had their expression induced after the inoculation with H. vastatrix, since the first time of sampling at 6 hours post-inoculation (hpi). At 24 hpi, there was a significant increase in expression, for most of the receptors evaluated, and at 48 hpi, a suppression. The results showed that the candidate sequences for PRRs in the C. arabica genome display high homology with fungal PRRs already described in the literature. Besides, they respond to pathogen inoculation and seem to be involved in the perception or signaling of fungal chitin, acting as receptors or co-receptors of this molecule. These findings represent an advance in the understanding of the basal immunity of this species.