72-Hour transport recovery of antimicrobial resistant Neisseria gonorrhoeae isolates using the InTray® GC method

• Published in: PloS one Vol. 17; no. 1; p. e0259668
• Main Authors: Paris, Keely S., Font, Brandon, Mehta, Sanjay R., Huerta, Irvin, Bristow, Claire C.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 21.01.2022; Public Library of Science (PLoS)
• Subjects: Antibiotics; Antiinfectives and antibacterials; Antimicrobial agents; Biology and Life Sciences; Clinical isolates; Drug resistance; Drug resistance in microorganisms; FDA approval; Gonorrhea; Health surveillance; Infectious diseases; Medicine and Health Sciences; Neisseria gonorrhoeae; Physical Sciences; Public health; Quality control; R&D; Recovery; Recovery (Medical); Research & development; Research and Analysis Methods; Testing; Transportation systems; United States; Oregon; La Jolla California; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0259668

Recovery of Neisseria gonorrhoeae isolates exposed to a range of transport times and temperatures was quantitatively assessed for two transport devices, BioMed Diagnostics’ InTray GC® and Copan Diagnostics’ Liquid Amies Elution Swab (ESwab®) Collection and Transport System. Respective devices were inoculated with N . gonorrhoeae , exposed to simulated transport conditions and spread-plated from serial dilutions in duplicates onto chocolate agar in order to count CFU (colony-forming units) in the range of 25–250. Baseline CFU/mL averages of time-zero transport for each device was compared to either 24 hour (Eswab) or 72 hour (InTray GC) CFU/mL average to assess recovery of six clinical isolates of N . gonorrhoeae , and differences showing no greater than a 3 log 10 (± 10%) decline between comparative time points qualified as acceptable. Our findings suggest that the InTray GC system has the potential to transport clinical isolates for ≤72 hours with acceptable N . gonorrhoeae recovery.