Inter-simple sequence repeat data reveals high genetic diversity in wild populations of the narrowly distributed endemic Lilium regale in the Minjiang River Valley of China

• Published in: PloS one Vol. 10; no. 3; p. e0118831
• Main Authors: Wu, Zhu-hua, Shi, Jisen, Xi, Meng-li, Jiang, Fu-xing, Deng, Ming-wen, Dayanandan, Selvadurai
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.03.2015; Public Library of Science (PLoS)
• Subjects: Analysis; Biodiversity; Biotechnology; Breeding; China; Conservation; Deoxyribonucleic acid; DNA; DNA, Plant - genetics; Education; Endangered & extinct species; Endemic plants; Evolution & development; Forestry; Gene Flow; Genetic diversity; Genetic variability; Genomics; Germplasm; Heterozygosity; Inbreeding; Laboratories; Landscape architecture; Lilium; Lilium - genetics; Lilium regale; Microsatellite Repeats; Moisture resistance; Polymorphism, Genetic; Population genetics; Populations; Rivers; Sequence Analysis, DNA; Species diversity; Studies; Wilderness; China; Canada; Montreal Quebec Canada; Japan; Quebec Canada; Taiwan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0118831

Lilium regale E.H. Wilson is endemic to a narrow geographic area in the Minjiang River valley in southwestern China, and is considered an important germplasm for breeding commercially valuable lily varieties, due to its vigorous growth, resistance to diseases and tolerance for low moisture. We analyzed the genetic diversity of eight populations of L. regale sampled across the entire natural distribution range of the species using Inter-Simple Sequence Repeat markers. The genetic diversity (expected heterozygosity= 0.3356) was higher than those reported for other narrowly distributed endemic plants. The levels of inbreeding (Fst = 0.1897) were low, and most of the genetic variability was found to be within (80.91%) than amongpopulations (19.09%). An indirect estimate of historical levels of gene flow (Nm =1.0678) indicated high levels of gene flow among populations. The eight analyzed populations clustered into three genetically distinct groups. Based on these results, we recommend conservation of large populations representing these three genetically distinct groups.