Exogenous melatonin promotes seed germination and osmotic regulation under salt stress in cotton (Gossypium hirsutum L.)

• Published in: PloS one Vol. 15; no. 1; p. e0228241
• Main Authors: Chen, Li, Liu, Liantao, Lu, Bin, Ma, Tongtong, Jiang, Dan, Li, Jin, Zhang, Ke, Sun, Hongchun, Zhang, Yongjiang, Bai, Zhiying, Li, Cundong
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 31.01.2020; Public Library of Science (PLoS)
• Subjects: Abiotic stress; Agronomy; Amines; Antioxidants; Biology and Life Sciences; Cell Membrane Permeability - drug effects; Cell membranes; Chlorides; Cotton; Crop growth; Ecology and Environmental Sciences; Electrolyte leakage; Electrolytes; Enzymes; Gene expression; Germination; Germination - drug effects; Gossypium - cytology; Gossypium - drug effects; Gossypium - growth & development; Gossypium - metabolism; Homeostasis; Hormones; Hydrogen; Hydrogen peroxide; Hydrogen storage; Instrument industry (Equipment); Laboratories; Life sciences; Lipids; Malondialdehyde; Malondialdehyde - metabolism; Melatonin; Melatonin - pharmacology; Membranes; Osmosis - drug effects; Peroxides; Physical Sciences; Physiological aspects; Physiological regulation; Physiology; Plant growth; Plant resistance; Proline; Proteins; Salinity tolerance; Salt Stress - drug effects; Salt tolerance; Seed germination; Seeds; Seeds - drug effects; Seeds - growth & development; Stress; Stress concentration; Sugar; Time; Japan; China; Beijing China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0228241

Melatonin (MT; N-acetyI-5-methoxytryptamine) is an amine hormone involved in abiotic stress resistance. Previous studies have confirmed that melatonin can promote seed germination, mediate physiological regulation mechanisms, and stimulate crop growth under stress. However, the osmotic regulation mechanism by which exogenous melatonin mediates salt tolerance in cotton is still largely unknown. To investigate the effect of salt stress on melatonin concentration in germinating cotton seeds, we analyzed melatonin content over time during seed germination under different treatments. Melatonin content reached its minimum at day 6, while cotton germination rates peaked at day 6, indicating melatonin content and seed germination are correlated. Then we investigated the effects of 10-100 μM melatonin treatments on membrane lipid peroxides and osmotic adjustment substances during cotton seed germination under salt stress. Salt stress led to electrolyte leakage (EL) as well as accumulations of hydrogen peroxide (H2O2), malondialdehyde (MDA), organic osmotic substances (i.e., proline, soluble sugars), and inorganic osmotic substances (i.e., Na+, Cl-). Meanwhile, the contents of melatonin, soluble proteins, and K+ as well as the K+/Na+ balance decreased, indicating that salt stress inhibited melatonin synthesis and damaged cellular membranes, seriously affecting seed germination. However, melatonin pretreatment at different concentrations alleviated the adverse effects of salt stress on cotton seeds and reduced EL as well as the contents of H2O2, MDA, Na+, and Cl-. The exogenous application of melatonin also promoted melatonin, soluble sugar, soluble proteins, proline, and K+/Na+ contents under salt stress. These results demonstrate that supplemental melatonin can effectively ameliorate the repression of cotton seed germination by enhancing osmotic regulating substances and adjusting ion homeostasis under salt stress. Thus, melatonin may potentially be used to protect cotton seeds from salt stress, with the 20 μM melatonin treatment most effectively promoting cotton seed germination and improving salt stress tolerance.