Beclin 1 and UVRAG confer protection from radiation-induced DNA damage and maintain centrosome stability in colorectal cancer cells

• Published in: PloS one Vol. 9; no. 6; p. e100819
• Main Authors: Park, Jae Myung, Tougeron, David, Huang, Shengbing, Okamoto, Koichi, Sinicrope, Frank A
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.06.2014; Public Library of Science (PLoS)
• Subjects: Apoptosis; Apoptosis - drug effects; Apoptosis - radiation effects; Apoptosis Regulatory Proteins - metabolism; Autophagy; Autophagy - drug effects; Autophagy - radiation effects; Autophagy-Related Protein 5; Beclin-1; Biology; Biology and life sciences; Cancer; Cancer genetics; Cancer prevention; Cancer therapies; Cell cycle; Cell death; Cell division; Cell Line, Tumor; Cell Nucleus - drug effects; Cell Nucleus - metabolism; Centrosome - drug effects; Centrosome - metabolism; Centrosome - radiation effects; Chemotherapy; Chromosomes; Clonal deletion; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Cytoprotection - drug effects; Cytoprotection - radiation effects; Cytotoxicity; Deletion mutant; Deoxyribonucleic acid; DNA; DNA Breaks, Double-Stranded - drug effects; DNA Breaks, Double-Stranded - radiation effects; DNA Damage; DNA repair; Fluorouracil - pharmacology; Gamma Rays; Gene Knockdown Techniques; Genomic instability; Homologous recombination; Homology; Humans; Hypoxia; Intracellular Signaling Peptides and Proteins - metabolism; Ionizing radiation; Irradiation; Kinases; Medicine and Health Sciences; Membrane Proteins - metabolism; Metabolism; Microtubule-Associated Proteins - metabolism; Phagocytosis; Protein Binding - drug effects; Protein Binding - radiation effects; Proteins; Rad51 Recombinase - metabolism; Radiation; Radiation damage; Radiation effects; siRNA; Stability; Tubulin; Tumor cells; Tumor Suppressor p53-Binding Protein 1; Tumor Suppressor Proteins - metabolism; Tumorigenesis; Tumors; Ultraviolet radiation
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0100819

Beclin 1 interacts with UV-irradiation-resistance-associated gene (UVRAG) to form core complexes that induce autophagy. While cells with defective autophagy are prone to genomic instability that contributes to tumorigenesis, it is unknown whether Beclin1 or UVRAG can regulate the DNA damage/repair response to cancer treatment in established tumor cells. We found that siRNA knockdown of Beclin 1 or UVRAG can increase radiation-induced DNA double strand breaks (DSBs), shown by pATM and γH2Ax, and promote colorectal cancer cell death. Furthermore, knockdown of Beclin 1, UVRAG or ATG5 increased the percentage of irradiated cells with nuclear foci expressing 53BP1, a marker of nonhomologous end joining but not RAD51 (homologous recombination), compared to control siRNA. Beclin 1 siRNA was shown to attenuate UVRAG expression. Cells with a UVRAG deletion mutant defective in Beclin 1 binding showed increased radiation-induced DSBs and cell death compared to cells with ectopic wild-type UVRAG. Knockdown of Beclin 1 or UVRAG, but not ATG5, resulted in a significant increase in centrosome number (γ-tubulin staining) in irradiated cells compared to control siRNA. Taken together, these data indicate that Beclin 1 and UVRAG confer protection against radiation-induced DNA DSBs and may maintain centrosome stability in established tumor cells.