Spatial distribution of epigenetic modifications in Brachypodium distachyon embryos during seed maturation and germination

• Published in: PloS one Vol. 9; no. 7; p. e101246
• Main Authors: Wolny, Elzbieta, Braszewska-Zalewska, Agnieszka, Hasterok, Robert
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.07.2014; Public Library of Science (PLoS)
• Subjects: Abscisic acid; Acetylation; Analysis; Arabidopsis; Arabidopsis thaliana; Barley; Biology and Life Sciences; Brachypodium; Brachypodium - growth & development; Brachypodium - physiology; Brachypodium distachyon; Cellular biology; Chromatin remodeling; Coleoptile; Deoxyribonucleic acid; Desiccants; Desiccation; DNA; DNA methylation; Drying; Embryonic development; Embryos; Environmental protection; Epigenesis, Genetic; Epigenetic inheritance; Epigenetics; Epithelial cells; Gene expression; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genomes; Germination; Grasses; Histones - metabolism; Hormones; Imbibition; Maturation; Methylation; Morphogenesis; Nuclei; Organs; Plant Proteins - metabolism; Primordia; Protein Processing, Post-Translational; Seeds; Seeds - growth & development; Seeds - physiology; Spatial distribution; Starch - metabolism; Tissues; Poland
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0101246

Seed development involves a plethora of spatially and temporally synchronised genetic and epigenetic processes. Although it has been shown that epigenetic mechanisms, such as DNA methylation and chromatin remodelling, act on a large number of genes during seed development and germination, to date the global levels of histone modifications have not been studied in a tissue-specific manner in plant embryos. In this study we analysed the distribution of three epigenetic markers, i.e. H4K5ac, H3K4me2 and H3K4me1 in 'matured', 'dry' and 'germinating' embryos of a model grass, Brachypodium distachyon (Brachypodium). Our results indicate that the abundance of these modifications differs considerably in various organs and tissues of the three types of Brachypodium embryos. Embryos from matured seeds were characterised by the highest level of H4K5ac in RAM and epithelial cells of the scutellum, whereas this modification was not observed in the coleorhiza. In this type of embryos H3K4me2 was most evident in epithelial cells of the scutellum. In 'dry' embryos H4K5ac was highest in the coleorhiza but was not present in the nuclei of the scutellum. H3K4me1 was the most elevated in the coleoptile but absent from the coleorhiza, whereas H3K4me2 was the most prominent in leaf primordia and RAM. In embryos from germinating seeds H4K5ac was the most evident in the scutellum but not present in the coleoptile, similarly H3K4me1 was the highest in the scutellum and very low in the coleoptile, while the highest level of H3K4me2 was observed in the coleoptile and the lowest in the coleorhiza. The distinct patterns of epigenetic modifications that were observed may be involved in the switch of the gene expression profiles in specific organs of the developing embryo and may be linked with the physiological changes that accompany seed desiccation, imbibition and germination.