Comparisons of the Effects of Elevated Vapor Pressure Deficit on Gene Expression in Leaves among Two Fast-Wilting and a Slow-Wilting Soybean

• Published in: PloS one Vol. 10; no. 10; p. e0139134
• Main Authors: Devi, Mura Jyostna, Sinclair, Thomas R, Taliercio, Earl
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.10.2015; Public Library of Science (PLoS)
• Subjects: Agricultural production; Analysis; Aquaporins; Arabidopsis; Biological Transport - genetics; Biosynthesis; Cell Wall - metabolism; Crop science; Crop yield; Drought; Drought conditions; Experiments; Gene expression; Gene Expression Profiling; Genes; Genetic aspects; Genotype; Genotypes; Glycine max; Glycine max - cytology; Glycine max - genetics; Glycine max - metabolism; Glycine max - physiology; Homeostasis; Humidity; Hydraulic conductivity; Hydrogeology; Influence; Kinetics; Leaves; Physiology; Plant growth; Plant Growth Regulators - metabolism; Plant Leaves - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Transpiration - genetics; Pressure; Pressure effects; Soybeans; Transcription Factors - genetics; Transcription Factors - metabolism; Transpiration; Vapor Pressure; Vapors; Water conservation; Water shortages; Wilting; North Carolina; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0139134

Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because these accessions have contrasting responses to VPD changes. Hutcheson, a fast-wilting soybean, and PI 471938, a slow-wilting soybean, respond to increased VPD with a linear increase in TR. TR of the slow-wilting PI 416937 is limited when VPD increases to greater than about 2 kPa. The objective of this study was to identify the response of the transcriptome of these accessions to elevated VPD under well-watered conditions and identify responses that are unique to the slow-wilting accessions. Gene expression analysis in leaves of genotypes PI 471938 and Hutcheson showed that 22 and 1 genes, respectively, were differentially expressed under high VPD. In contrast, there were 944 genes differentially expressed in PI 416937 with the same increase in VPD. The increased alteration of the transcriptome of PI 416937 in response to elevated VPD clearly distinguished it from the other slow-wilting PI 471938 and the fast-wilting Hutcheson. The inventory and analysis of differentially expressed genes in PI 416937 in response to VPD is a foundation for further investigation to extend the current understanding of plant hydraulic conductivity in drought environments.