MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection

• Published in: PloS one Vol. 13; no. 3; p. e0193709
• Main Authors: Zekri, Abdel-Rahman N, El-Sisi, Enas Reda, Youssef, Amira Salah El-Din, Kamel, Mahmoud M, Nassar, Auhood, Ahmed, Ola Sayed, El Kassas, Mohamed, Barakat, Ahmed Barakat, Abd El-Motaleb, Alaa Ismail, Bahnassy, Abeer A
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.03.2018; Public Library of Science (PLoS)
• Subjects: Apoptosis; Biology; Biology and life sciences; Blood; Bone marrow; Care and treatment; Cell adhesion & migration; Cell growth; Cirrhosis; Development and progression; Gene expression; Genetic aspects; Health aspects; Hepatitis; Hepatitis C; Hepatocellular carcinoma; Identification methods; Immunology; Infections; Liver; Liver cancer; Liver cirrhosis; Long bone; Medical prognosis; Medicine and Health Sciences; MicroRNA; MicroRNAs; miRNA; Patients; Peripheral blood; Research and Analysis Methods; Ribonucleic acid; RNA; Stem cells; Virology; Cairo Egypt; Egypt
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0193709

Molecular characterization of the CD133+ stem cells associated with hepatocarinogensis through identifying the expression patterns of specific microRNAs (miRNAs). We investigated the expression pattern of 13 miRNAs in purified CD133+ cells separated from the peripheral blood of healthy volunteers, chronic hepatitis C (CHC), liver cirrhosis (LC) and hepatocellular carcinoma (HCC) patients a long with bone marrow samples from the healthy volunteers and the LC patients using custom miScript miRNA PCR array. The differential expression of the 13 studied miRNAs in CD133+ cells separated from the HCC patients' peripheral blood compared to the controls revealed that miR-602, miR-181b, miR-101, miR-122, miR-192, miR-125a-5p, and miR-221 were significantly up regulated (fold change = 1.8, 1.7, 2, 5.4, 1.6, 2.9 & 1.5 P value = 0.039, 0.0019, 0.0013, 0.0370, 00024, 0.000044 &0.000007 respectively). As for the HCC group compared to the CHC group; miR-602, miR-122, miR-181b, miR-125a-5p, and miR-192 were significantly up regulated (fold change = 13, 3.1, 2.8, 1.6 & 1.56, P value = 0.01, 0.001, 0.000004, 0.002 & 0.007 respectively). Upon comparing the HCC group to the LC group; miR-199a-3p, miR-192, miR-122, miR-181b, miR-224, miR-125a-5p, and miR-885-5p were significantly up regulated (fold change = 5, 6.7, 2.3, 3, 2.5, 4.2 & 39.5 P value = 0.001025, 0.000024, 0.000472, 0.000278, 0.000004, 0.000075 & 0.0000001 respectively) whereas miR-22 was significantly down regulated (fold change = 0.57 P value = 0.00002). Only, miR-192, miR-122, miR-181b and miR-125a-5p were significant common miRNAs in CD133+ cells of the HCC group compared to the other non-malignant groups. We identified a miRNA panel comprised of four miRNAs (miR-192, miR-122, miR-181b and miR-125a-5p) that may serve as a molecular tool for characterization of the CD133+ cells associated with different stages of hepatocarinogensis. This panel may aid in developing a new target therapy specific for those CD133+ cells.