Clinical significance of peripheral blood and tumor tissue lymphocyte subsets in cervical cancer patients

• Published in: BMC cancer Vol. 20; no. 1; p. 173
• Main Authors: Wu, Yutuan, Ye, Shuang, Goswami, Shyamal, Pei, Xuan, Xiang, Libing, Zhang, Xiaoming, Yang, Huijuan
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.03.2020; BioMed Central; BMC
• Subjects: Cancer patients; Cancer vaccines; Care and treatment; Cervical cancer; Cervix; Clinical significance; Clinicopathological variables; Dendritic cells; Disease; Disease susceptibility; Flow cytometry; Granulocytes; Gynecology; Human papillomavirus; Immune system; Killer cells; Lymphocyte subsets; Lymphocytes; Patients; Peripheral blood; Prognosis; Software; Surgery; T cells; Tumor immunology; Tumors; United States > US; Prognosis; Lymphocyte subsets; Clinicopathological variables; Cervical cancer; Tumor immunology
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-020-6633-x

Alterations in peripheral blood lymphocytes in cervical cancer have been reported, although conflicting views exist. The present study investigated the distributions of lymphocyte subsets in tumor tissue and peripheral blood samples from cervical cancer patients and precancerous lesion patients, and evaluated the correlations of lymphocyte subsets with clinicopathological and prognostic variables. A total of 44 patients with stage IB1-IIA2 cervical cancer and 13 precancerous lesion patients were included. Lymphocytes were collected from the tumor tissue and the peripheral blood, and isolated by Lymphoprep density gradient centrifugation. The percentages of lymphocyte subsets were quantified by flow cytometry analysis, and the differences between lymphocyte subsets in the tumor tissue and peripheral blood were compared by SPSS. In addition, the relationships between lymphocyte subsets and clinicopathological and prognostic variables were analyzed. Our results revealed that the amount of total T lymphocytes, CD8+ T cells, granulocytes, pDCs, CD16+ monocytes and CD56 NK cells were significantly higher in the tumor tissue than in the peripheral blood in the cervical cancer patients, while those of CD4+ T cells, CD4+/CD8+ cell ratio, rdT cells, BDCA1+ mDCs, total monocytes, CD14+ monocytes, NK cells and CD56 NK cells exhibited the opposite trend (p < 0.05). The levels of total pDCs and BDCA1+ mDCs in the peripheral blood were significantly lower in the cervical cancer patients than in the precancerous lesion patients, while the proportion of CD16+ monocytes was elevated (p < 0.05). In addition, some lymphocyte subsets, especially CD4+ cells and CD8+ cells, and the CD4+/CD8+ cell ratio were closely associated with clinicopathological and prognostic parameters. These results suggested that distinct alterations in infiltrating lymphocyte subsets occurred in the tumor and were associated with clinicopathological and prognostic parameters. Systemic impairment of the immune system may occur in the antitumor response of cervical cancer patients.