Evidence for divergent evolution of growth temperature preference in sympatric Saccharomyces species

• Published in: PloS one Vol. 6; no. 6; p. e20739
• Main Authors: Gonçalves, Paula, Valério, Elisabete, Correia, Cláudia, de Almeida, João M G C F, Sampaio, José Paulo
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02.06.2011; Public Library of Science (PLoS)
• Subjects: Adaptation, Physiological; Analysis; Baking yeast; Bark; Biological evolution; Biology; Cold Temperature; Divergence; Divergent evolution; Domestication; Enzymatic activity; Evolution; Evolution, Molecular; Evolutionary genetics; Fluxes; Fungal Proteins - metabolism; Genes; Genome, Fungal - genetics; Genomes; Genomics; Glycolysis; Growth; Habitats; Hypotheses; Mutation; Preferences; Proteins; Saccharomyces; Saccharomyces - genetics; Saccharomyces - growth & development; Saccharomyces - metabolism; Saccharomyces - physiology; Saccharomyces cerevisiae; Selection, Genetic; Speciation; Species comparisons; Species Specificity; Sugar; Sympatric populations; Sympatry; Temperature; Temperature effects; Temperature preferences; Temperature profiles; Yeast; North America; Portugal
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0020739

The genus Saccharomyces currently includes eight species in addition to the model yeast Saccharomyces cerevisiae, most of which can be consistently isolated from tree bark and soil. We recently found sympatric pairs of Saccharomyces species, composed of one cryotolerant and one thermotolerant species in oak bark samples of various geographic origins. In order to contribute to explain the occurrence in sympatry of Saccharomyces species, we screened Saccharomyces genomic data for protein divergence that might be correlated to distinct growth temperature preferences of the species, using the dN/dS ratio as a measure of protein evolution rates and pair-wise species comparisons. In addition to proteins previously implicated in growth at suboptimal temperatures, we found that glycolytic enzymes were among the proteins exhibiting higher than expected divergence when one cryotolerant and one thermotolerant species are compared. By measuring glycolytic fluxes and glycolytic enzymatic activities in different species and at different temperatures, we subsequently show that the unusual divergence of glycolytic genes may be related to divergent evolution of the glycolytic pathway aligning its performance to the growth temperature profiles of the different species. In general, our results support the view that growth temperature preference is a trait that may have undergone divergent selection in the course of ecological speciation in Saccharomyces.