Gene expression of ANP, BNP and ET-1 in the heart of rats during pulmonary embolism

• Published in: PloS one Vol. 5; no. 6; p. e11111
• Main Authors: Gutte, Henrik, Oxbøl, Jytte, Kristoffersen, Ulrik Sloth, Mortensen, Jann, Kjaer, Andreas
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.06.2010; Public Library of Science (PLoS)
• Subjects: Animals; Atrial Natriuretic Factor - genetics; Atrium; Base Sequence; Biomarkers; Brain; Brain natriuretic peptide; Calcium-binding protein; Cardiovascular Disorders/Hemodynamics; Cardiovascular Disorders/Venous Thromboembolism; Chambers; DNA Primers; Embolism; Embolisms; Endothelin; Endothelin 1; Endothelin-1 - genetics; Gene Expression; Genes; Health sciences; Heart; Heart diseases; Heart failure; Hormones; Hospitals; Male; Microparticles; Mortality; Myocardium - metabolism; Natriuretic Peptide, Brain - genetics; Natriuretic peptides; Nuclear medicine; Peptides; Pets; Physiology; Physiology/Cardiovascular Physiology and Circulation; Plasma levels; Plasmas (physics); Polymerase Chain Reaction; Polystyrene; Polystyrene resins; Pulmonary arteries; Pulmonary embolism; Pulmonary Embolism - genetics; Pulmonary embolisms; Pulmonary hypertension; Rats; Rats, Sprague-Dawley; Respiratory diseases; Rodents; Secretion; Thromboembolism; Troponin; Troponin I; Ventricle; Ventricles (cerebral); Denmark; Germany
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0011111

Atrial natriuretic petide (ANP), brain natriuretic peptide (BNP) and endothelin-1 (ET-1) may reflect the severity of right ventricular dysfunction (RVD) in patients with pulmonary embolism (PE). The exact nature and source of BNP, ANP and ET-1 expression and secretion following PE has not previously been studied. Polystyrene microparticles were injected to induce PE in rats. Gene expression of BNP, ANP and ET-1 were determined in the 4 cardiac chambers by quantitative real time polymerase chain reaction (QPCR). Plasma levels of ANP, BNP, ET-1 and cardiac troponin I (TNI) were measured in plasma. PE dose-dependently increased gene expression of ANP and BNP in the right ventricle (RV) and increased gene expression of ANP in the right atrium (RA). In contrast PE dose-dependently decreased BNP gene expression in both the left ventricle (LV) and the left atrium (LA). Plasma levels of BNP, TNI and ET-1 levels dose-dependently increased with the degree of PE. We found a close correlation between PE degree and gene-expression of ANP, and BNP in the cardiac chambers with a selective increase in the right chambers of the heart. The present data supports the idea of natriuretic peptides as valuable biomarkers of RVD in PE.