Improvement of the antitumor activity of black currant [Ribes nigrum] polysaccharide by an enzymatic treatment

• Published in: Bioscience, biotechnology, and biochemistry Vol. 71; no. 5; pp. 1342 - 1344
• Main Authors: Takata, R.(Mercian Corp., Fujisawa, Kanagawa (Japan)), Yanai, T, Yamamoto, R, Konno, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.05.2007; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: AGENTES ANTINEOPLASTICOS; Animals; ANTINEOPLASTIC AGENTS; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; antitumor; Aspergillus oryzae; Aspergillus oryzae - enzymology; beta-Galactosidase - pharmacology; BIOCHEMICAL ENGINEERING; Biological and medical sciences; black currant; Buffers; Carcinoma, Ehrlich Tumor - drug therapy; Cassis; Cell Line; Chromatography, High Pressure Liquid; Dose-Response Relationship, Drug; ENZIMAS; ENZYME; ENZYMES; Fundamental and applied biological sciences. Psychology; GALACTOSIDASAS; GALACTOSIDASE; GALACTOSIDASES; GENIE BIOCHIMIQUE; Hydrogen-Ion Concentration; INGENIERIA BIOQUIMICA; MACROFAGOS; MACROPHAGE; Macrophage Activation - drug effects; MACROPHAGES; Macrophages, Peritoneal - drug effects; MEDICAMENT CYTOSTATIQUE; Mice; Mice, Inbred ICR; Molecular Weight; Neoplasm Transplantation; Phosphates - chemistry; POLISACARIDOS; POLYHOLOSIDE; polysaccharide; POLYSACCHARIDES; Polysaccharides - chemistry; Polysaccharides - metabolism; Polysaccharides - pharmacology; Reference Standards; Ribes - chemistry; RIBES NIGRUM; Temperature; Time Factors; Transplantation, Homologous; β-galactosidase; Antineoplastic agent; black currant; Enzymatic treatment; Enzyme; antitumor; Glycosidases; Hydrolases; O-Glycosidases; Polysaccharide; Optimization; β-Galactosidase; Macrophage
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.60725

A polysaccharide-rich substance isolated from black currant, named cassis polysaccharide (CAPS), was partially digested with beta-galactosidase from Aspergillus oryzae and its immunostimulatory activity was investigated. The in vitro cytokine-inducing effect of CAPS on RAW264 cells was gradually decreased along with lowering of the average MW of CAPS. In vivo, partially digested CAPS with a mean MW of approximately 20000 showed the most potent antitumor activity against Ehrlich carcinoma in mice.