Characterization of Glycoproteins in Equine Herpesvirus-1

• Published in: Journal of Veterinary Medical Science Vol. 75; no. 10; pp. 1317 - 1321
• Main Authors: Hassan Y. A. H. MAHMOUD, ANDOH, Kiyohiko, HATTORI, Shiho, TERADA, Yutaka, NOGUCHI, Keita, SHIMODA, Hiroshi, MAEDA, Ken
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2013; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Antibodies, Monoclonal - immunology; Cell Line; equine herpesvirus-1; Female; glycoprotein; Glycoproteins - genetics; Glycoproteins - immunology; Herpesviridae Infections - immunology; Herpesviridae Infections - veterinary; Herpesviridae Infections - virology; Herpesvirus 1, Equid - genetics; Herpesvirus 1, Equid - immunology; Horse Diseases - immunology; Horse Diseases - virology; Horses; Immunoblotting - veterinary; Mice; Mice, Inbred BALB C; monoclonal antibody; Neutralization Tests - veterinary; Plasmids - genetics; Plasmids - immunology; Virology
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.13-0168

In this study, we attempted to express twelve glycoproteins of equine herpesvirus-1 (EHV-1) in 293T cells and to characterize these using monoclonal antibodies (MAbs) and horse sera against EHV-1. Expression of glycoprotein B (gB), gC, gD, gG, gI and gp2 was recognized by immunoblot analysis using horse sera, but that of gE, gH, gK, gL, gM and gN was not. Four MAbs recognized gB, four recognized gC and one recognized gp2. Two MAbs against gB cross-reacted with EHV-4. Interestingly, coexpression of gE and gI and gM and gN enhanced their antigenicity. Furthermore, immunoblot analysis of gp2 showed that different molecular masses of gp2 were recognized by the MAb against gp2 and horse sera against EHV-1. In this study, it was demonstrated that at least six glycoproteins were immunogenic to horses, and coexpression of gE and gI and gM and gN was important for enhancement of antigenicity.