Analysis of lipoprotein profiles of healthy cats by gel-permeation high-performance liquid chromatography

• Published in: Journal of Veterinary Medical Science Vol. 78; no. 8; pp. 1277 - 1281
• Main Authors: MIZUTANI, Hisashi, SAKO, Toshinori, OKUDA, Hiroko, ARAI, Nobuaki, KURIYAMA, Koji, MORI, Akihiro, YOSHIMURA, Itaru, KOYAMA, Hidekazu
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2016; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; cat; Cats; Cats - blood; Cats - metabolism; Cholesterol; Cholesterol - blood; Chromatography; Chromatography, High Pressure Liquid - veterinary; Chylomicrons - blood; Density; Electrophoresis; Electrophoresis, Agar Gel - veterinary; Feasibility studies; Gel electrophoresis; High density lipoprotein; High performance liquid chromatography; HPLC; Internal Medicine; lipoprotein profile; Lipoproteins; Lipoproteins (very low density); Lipoproteins - blood; Lipoproteins, HDL - blood; Lipoproteins, LDL - blood; Lipoproteins, VLDL - blood; Liquid chromatography; Low density lipoprotein; Male; Males; On-line systems; triglyceride; Ultracentrifugation
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.16-0052

Density gradient ultracentrifugation (DGUC) and gel electrophoresis are conventionally used to obtain lipoprotein profiles of animals. We recently applied high-performance liquid chromatography with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system to dogs for lipoprotein profile analysis. We compared the GP-HPLC with DGUC as a method to obtain a feline lipoprotein profile. The lipoprotein profiles showed large and small peaks, which corresponded to high-density lipoprotein (HDL) and low-density lipoprotein (LDL), respectively, whereas very low-density lipoprotein (VLDL) and chylomicron (CM) were only marginally detected. This profile was very similar to that of dogs reported previously. Healthy cats also had a small amount of cholesterol-rich particles distinct from the normal LDL or HDL profile. There was no difference in lipoprotein profiles between the sexes, but males had a significantly larger LDL particle size (P=0.015). This study shows the feasibility of GP-HPLC for obtaining accurate lipoprotein profiles with small sample volumes and provides valuable reference data for healthy cats that should facilitate diagnoses.