Determination of Iridoid Glucosides for Quality Assessment of Herba Oldenlandiae by High-Performance Liquid Chromatography

Herba Oldenlandiae, the dried herb of Oldenlandia diffusa (WILLD.) ROXB. (Family Rubiaceae), is officially listed in the Chinese Pharmacopoeia. In the herbal market, two substitutes originated from O. corymbosa (L.) LAM and O. tenelliflora BL. are commonly used. In light of this, the target in setti...

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Published inChemical & Pharmaceutical Bulletin Vol. 54; no. 8; pp. 1131 - 1137
Main Authors Liang, Zhi-Tao, Jiang, Zhi-Hong, Leung, Kelvin Sze-Yin, Zhao, Zhong-Zhen
Format Journal Article
LanguageEnglish
Japanese
Published TOKYO The Pharmaceutical Society of Japan 2006
Pharmaceutical Society of Japan
Pharmaceutical Soc Japan
Japan Science and Technology Agency
Subjects
Chemistry
Chemistry, Medicinal
Chemistry, Multidisciplinary
Chromatography, High Pressure Liquid
Herba Oldenlandiae
HPLC
iridoid glucoside
Iridoids - analysis
Life Sciences & Biomedicine
Medicine, Chinese Traditional
Molecular Structure
Oldenlandia
Oldenlandia - chemistry
Pharmacology & Pharmacy
Physical Sciences
Plants, Medicinal - chemistry
Quality Control
Science & Technology
FRUITS
Herba Oldenlandiae
ANTIINFLAMMATORY ACTIVITY
INHIBITION
quality control
GLYCOSIDE
Oldenlandia
HEDYOTIS-DIFFUSA
iridoid glucoside
HPLC
CONSTITUENTS
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Summary:Herba Oldenlandiae, the dried herb of Oldenlandia diffusa (WILLD.) ROXB. (Family Rubiaceae), is officially listed in the Chinese Pharmacopoeia. In the herbal market, two substitutes originated from O. corymbosa (L.) LAM and O. tenelliflora BL. are commonly used. In light of this, the target in setting up a method for quality assessment of Herba Oldenlandiae is urgently needed. In this article, a simple and reliable high-performance liquid chromatographic (HPLC) method was developed for quantifying asperuloside (1), E-6-O-p-coumaroyl scandoside methyl ester (2) and E-6-O-p-coumaroyl scandoside methyl ester-10-methyl ether (3) in Herba Oldenlandiae derived from O. diffusa. Among them, compound 3 is a new compound isolated from O. diffusa. All these unique compounds were used as markers for the first time in the quality assessment of Herba Oldenlandiae. The results showed that the contents of compounds 1—3 were significantly varied among different samples whilst those of compounds 2 and 3 were found to be lower in contents in the two substitutes of O. diffusa. The analytical method is suitable for quality control of Herba Oldenlandiae and useful in differentiation from its confusable species. The method has been fully validated with satisfactory linearity, accuracy, precision and stability.
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ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.54.1131