Enhanced recombinant protein production and differential expression of molecular chaperones in sf-caspase-1-repressed stable cells after baculovirus infection

• Published in: BMC biotechnology Vol. 12; no. 1; p. 83
• Main Authors: Lai, Yiu-Kay, Hsu, John T-A, Chu, Chih-Chieh, Chang, Teng-Yuan, Pan, Kao-Lu, Lin, Chih-Chien
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 07.11.2012; BioMed Central; BMC
• Subjects: Animals; Apoptosis; Baculoviridae - genetics; Baculovirus; Baculoviruses; Caspases - chemistry; Caspases - genetics; Caspases - metabolism; Chaperone; Development and progression; DNA virus infections; Enzymes; Genes; Genetic aspects; Health aspects; Luciferase; Medical research; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; Phosphatases; Physiological aspects; Plasmids - genetics; Plasmids - metabolism; Proteins; Recombinant proteins; Recombinant Proteins - biosynthesis; Recombinant Proteins - genetics; RNA; RNA Interference; Sf-caspase-1; Sf9 Cells; Spodoptera; Spodoptera frugiperda; Transfection; Taiwan
• ISSN: 1472-6750; 1472-6750
• DOI: 10.1186/1472-6750-12-83

There are few studies that have examined the potential of RNA inference (RNAi) to increase protein production in the baculovirus expression vector system (BEVS). Spodoptera frugiperda (fall armyworm) (Sf)-caspase-1-repressed stable cells exhibit resistance to apoptosis and enhancement of recombinant protein production. However, the mechanism of recombinant protein augmentation in baculovirus-infected Caspase-repressed insect cells has not been elucidated. In the current study, we utilized RNAi-mediated Sf-caspase-1-repressed stable cells to clarify how the resistance to apoptosis can enhance both intracellular (firefly luciferase) and extracellular (secreted alkaline phosphatase [SEAP]) recombinant protein production in BEVS. Since the expression of molecular chaperones is strongly associated with the maximal production of exogenous proteins in BEVS, the differential expression of molecular chaperones in baculovirus-infected stable cells was also analyzed in this study. The data indicated that the retention of expression of molecular chaperones in baculovirus-infected Sf-caspase-1-repressed stable cells give the higher recombinant protein accumulation.