Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an...

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Published inJournal of Veterinary Medical Science Vol. 78; no. 2; pp. 309 - 311
Main Authors BANNAI, Hiroshi, NEMOTO, Manabu, TSUJIMURA, Koji, YAMANAKA, Takashi, MAEDA, Ken, KONDO, Takashi
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.2016
Japan Science and Technology Agency
The Japanese Society of Veterinary Science
Subjects
Amino Acid Sequence
Animals
Antigens, Viral - immunology
Chemistry Techniques, Synthetic
EHV-4
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Equine herpesvirus
glycoprotein G
Herpesvirus 4, Equid - isolation & purification
Horse Diseases - virology
Horses
peptide ELISA
Peptide Fragments - chemical synthesis
Peptide Fragments - immunology
Sensitivity and Specificity
Viral Envelope Proteins - chemical synthesis
Viral Envelope Proteins - immunology
Virology
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Abstract To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.
AbstractList To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.
To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [ 4 ], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.
Author TSUJIMURA, Koji
BANNAI, Hiroshi
NEMOTO, Manabu
MAEDA, Ken
KONDO, Takashi
YAMANAKA, Takashi
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  organization: Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400–4 Shiba, Shimotsuke, Tochigi 329–0412, Japan
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10.1128/JCM.42.3.1095-1098.2004
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References 7. Yasunaga, S., Maeda, K., Matsumura, T., Kai, K., Iwata, H. and Inoue, T. 1998. Diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in Japan using a type-specific ELISA. J. Vet. Med. Sci. 60: 1133–1137.
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1318750 - J Vet Med Sci. 1992 Apr;54(2):207-11
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– reference: 5. Maeda, K., Mizukoshi, F., Hamano, M., Kai, K., Kondo, T. and Matsumura, T. 2005. Identification of another B-cell epitope in the type-specific region of equine herpesvirus 4 glycoprotein G. Clin. Diagn. Lab. Immunol. 12: 122–124.
– reference: 3. Landis, J. R. and Koch, G. G. 1977. The measurement of observer agreement for categorical data. Biometrics 33: 159–174.
– reference: 7. Yasunaga, S., Maeda, K., Matsumura, T., Kai, K., Iwata, H. and Inoue, T. 1998. Diagnosis and sero-epizootiology of equine herpesvirus type 1 and type 4 infections in Japan using a type-specific ELISA. J. Vet. Med. Sci. 60: 1133–1137.
– reference: 4. Maeda, K., Mizukoshi, F., Hamano, M., Kai, K., Iwata, H., Kondo, T. and Matsumura, T. 2004. Development of an equine herpesvirus type 4-specific enzyme-linked immunosorbent assay using a B-cell epitope as an antigen. J. Clin. Microbiol. 42: 1095–1098.
– reference: 6. Matsumura, T., Sugiura, T., Imagawa, H., Fukunaga, Y. and Kamada, M. 1992. Epizootiological aspects of type 1 and type 4 equine herpesvirus infections among horse populations. J. Vet. Med. Sci. 54: 207–211.
– reference: 2. Crabb, B. S. and Studdert, M. J. 1993. Epitopes of glycoprotein G of equine herpesviruses 4 and 1 located near the C termini elicit type-specific antibody responses in the natural host. J. Virol. 67: 6332–6338.
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  doi: 10.1128/JVI.67.10.6332-6338.1993
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  doi: 10.1292/jvms.60.1133
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  doi: 10.1292/jvms.54.207
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  doi: 10.2307/2529310
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  doi: 10.1007/978-1-4613-1587-2_6
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  doi: 10.1128/JCM.42.3.1095-1098.2004
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  doi: 10.1128/CDLI.12.1.122-124.2005
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– reference: 9819768 - J Vet Med Sci. 1998 Oct;60(10):1133-7
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– reference: 7690425 - J Virol. 1993 Oct;67(10):6332-8
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Snippet To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G...
To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G...
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SubjectTerms Amino Acid Sequence
Animals
Antigens, Viral - immunology
Chemistry Techniques, Synthetic
EHV-4
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Equine herpesvirus
glycoprotein G
Herpesvirus 4, Equid - isolation & purification
Horse Diseases - virology
Horses
peptide ELISA
Peptide Fragments - chemical synthesis
Peptide Fragments - immunology
Sensitivity and Specificity
Viral Envelope Proteins - chemical synthesis
Viral Envelope Proteins - immunology
Virology
Title Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen
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