Improvement of an enzyme-linked immunosorbent assay for equine herpesvirus type 4 by using a synthetic-peptide 24-mer repeat sequence of glycoprotein G as an antigen

• Published in: Journal of Veterinary Medical Science Vol. 78; no. 2; pp. 309 - 311
• Main Authors: BANNAI, Hiroshi, NEMOTO, Manabu, TSUJIMURA, Koji, YAMANAKA, Takashi, MAEDA, Ken, KONDO, Takashi
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.2016; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Amino Acid Sequence; Animals; Antigens, Viral - immunology; Chemistry Techniques, Synthetic; EHV-4; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Equine herpesvirus; glycoprotein G; Herpesvirus 4, Equid - isolation & purification; Horse Diseases - virology; Horses; peptide ELISA; Peptide Fragments - chemical synthesis; Peptide Fragments - immunology; Sensitivity and Specificity; Viral Envelope Proteins - chemical synthesis; Viral Envelope Proteins - immunology; Virology
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.15-0275

To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4 (EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer: MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%), whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer ELISA.