Protective Effects of Panduratin A against Oxidative Damage of tert-Butylhydroperoxide in Human HepG2 Cells

The protective effect of panduratin A, isolated from Kaempferia pandurata ROXB. (Zingiberaceae), against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity was investigated in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazo...

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Bibliographic Details
Published inBiological & Pharmaceutical Bulletin Vol. 28; no. 6; pp. 1083 - 1086
Main Authors Sohn, Jong Hee, Han, Kyu-Lee, Lee, Sun-Hee, Hwang, Jae-Kwan
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 01.06.2005
Pharmaceutical Society of Japan
Japan Science and Technology Agency
Subjects
Antioxidants - isolation & purification
Antioxidants - pharmacology
Cell Line, Tumor
Chalcones
HepG2 cell
Humans
oxidative damage
Oxidative Stress - drug effects
Oxidative Stress - physiology
panduratin A
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Rhizome
tert-butylhydroperoxide
tert-Butylhydroperoxide - antagonists & inhibitors
tert-Butylhydroperoxide - toxicity
Zingiberaceae
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Summary:The protective effect of panduratin A, isolated from Kaempferia pandurata ROXB. (Zingiberaceae), against tert-butylhydroperoxide (t-BHP)-induced cytotoxicity was investigated in a human hepatoma cell line, HepG2. The tetrazolium dye colorimetric test (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay) was used to monitor cytotoxicity. Lipid peroxidation [malondialdehyde (MDA) formation] and intracellular glutathione level were estimated by fluorometric methods. Intracellular reactive oxygen species (ROS) formation was measured using a fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA). Panduratin A significantly reduced the cell growth inhibition caused by t-BHP. Furthermore, panduratin A ameliorated lipid peroxidation as demonstrated by a reduction in MDA formation, and attenuated glutathione (GSH) depletion in a dose-dependent manner. It was also found that panduratin A reduced intracellular ROS formation caused by t-BHP. These results strongly suggest that panduratin A has significant protective ability against oxidative damage caused by reactive intermediates.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.28.1083