Differentiation of Rhizoma Et Radix Polygoni Cuspidati from Closely Related Herbs by HPLC Fingerprinting

• Published in: Chemical & Pharmaceutical Bulletin Vol. 54; no. 8; pp. 1179 - 1186
• Main Authors: Qian, Guangsheng, Leung, Sik-Yuen, Lu, GuangHua, Leung, Kelvin Sze-Yin
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Pharmaceutical Society of Japan 2006; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Chromatography, High Pressure Liquid - methods; Chromatography, High Pressure Liquid - standards; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - classification; Fallopia japonica - chemistry; Fallopia japonica - classification; fingerprint; HPLC-DAD-MS; Molecular Structure; Polygonaceae; Quality Control; Rhizoma Et Radix Polygoni Cuspidati; Rhizome - chemistry; Technology, Pharmaceutical - methods
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.54.1179

An HPLC-DAD fingerprinting profile of Rhizoma Et Radix Polygoni Cuspidati was established basing on the consistent chromatographic features of 24 authentic herb samples. The major types of chemical constituents, stilbenes and anthraquinones, were analyzed and included in the fingerprint. Eight common peaks of Polygonum Cuspidatum were identified by using HPLC-MS. The developed fingerprint was applied to differentiate Rhizoma Et Radix Polygoni Cuspidati from Radix Polygoni Multiflori and Radix Et Rhizoma Rhei. Although the three herbs belong to the family of Polygonaceae, the results indicated that these could be differentiated by using the established method.