Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene

• Published in: Journal of Veterinary Medical Science Vol. 76; no. 1; pp. 119 - 122
• Main Authors: WATANABE, Masashi, TANAKA, Kazuaki, TAKIZAWA, Tatsuya, SEGAWA, Kazuhito, NEO, Sakurako, TSUCHIYA, Ryo, MURATA, Michiko, MURAKAMI, Masaru, HISASUE, Masaharu
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.01.2014; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Base Sequence; Biochemistry; canine TNFA gene; DNA - chemistry; DNA - genetics; Dogs - genetics; Introns - genetics; Microsatellite Repeats - genetics; Molecular Sequence Data; Polymerase Chain Reaction - veterinary; Polymorphism, Single Nucleotide - genetics; Sequence Alignment; Sequence Analysis, DNA; tetranucleotide microsatellite; Tumor Necrosis Factor-alpha - genetics
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.13-0316

A polymorphic tetranucleotide (GAAT)n microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)5, (GAAT)6, (GAAT)7 and (GAAT)8, including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene.