Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2
Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol...
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| Published in | Emerging infectious diseases Vol. 26; no. 7; pp. 1633 - 1635 |
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| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
U.S. National Center for Infectious Diseases
01.07.2020
Centers for Disease Control and Prevention |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands. |
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| AbstractList | Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands. Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands. |
| Audience | Professional Academic |
| Author | Stittleburg, Victoria Saklawi, Youssef Waggoner, Jesse J. Babiker, Ahmed Hussaini, Laila Rouphael, Nadine Anderson, Evan J. Sahoo, Malaya K. Pinsky, Benjamin A. Kraft, Colleen S. Pond, Renee |
| Author_xml | – sequence: 1 givenname: Jesse J. surname: Waggoner fullname: Waggoner, Jesse J. – sequence: 2 givenname: Victoria surname: Stittleburg fullname: Stittleburg, Victoria – sequence: 3 givenname: Renee surname: Pond fullname: Pond, Renee – sequence: 4 givenname: Youssef surname: Saklawi fullname: Saklawi, Youssef – sequence: 5 givenname: Malaya K. surname: Sahoo fullname: Sahoo, Malaya K. – sequence: 6 givenname: Ahmed surname: Babiker fullname: Babiker, Ahmed – sequence: 7 givenname: Laila surname: Hussaini fullname: Hussaini, Laila – sequence: 8 givenname: Colleen S. surname: Kraft fullname: Kraft, Colleen S. – sequence: 9 givenname: Benjamin A. surname: Pinsky fullname: Pinsky, Benjamin A. – sequence: 10 givenname: Evan J. surname: Anderson fullname: Anderson, Evan J. – sequence: 11 givenname: Nadine surname: Rouphael fullname: Rouphael, Nadine |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32294051$$D View this record in MEDLINE/PubMed |
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| References | Wang (key-10.3201/eid2607.201285-202006181313-R1) 2020 key-10.3201/eid2607.201285-202006181313-R2 Babiker (key-10.3201/eid2607.201285-202006181313-R7) 2020 key-10.3201/eid2607.201285-202006181313-R8 key-10.3201/eid2607.201285-202006181313-R9 Chan (key-10.3201/eid2607.201285-202006181313-R5) 2020 Chu (key-10.3201/eid2607.201285-202006181313-R3) 2020; 66 Shirato (key-10.3201/eid2607.201285-202006181313-R6) 2020 Rojas (key-10.3201/eid2607.201285-202006181313-R10) 2018; 98 Corman (key-10.3201/eid2607.201285-202006181313-R4) 2020; 25 |
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| SubjectTerms | Betacoronavirus - genetics Betacoronavirus - isolation & purification Coronaviruses COVID-19 Disease control Disease prevention Expedited Genes Humans Infections Medical laboratories molecular detection Multiplex Polymerase Chain Reaction - methods Nasopharynx - virology Pathogens Real-Time Polymerase Chain Reaction - methods Research Letter respiratory infections RNA RNA polymerase rRT-PCR SARS-CoV-2 Severe acute respiratory syndrome Severe acute respiratory syndrome coronavirus 2 Transcription (Genetics) Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2 Viral infections Viruses |
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| Title | Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2 |
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