Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2

• Published in: Emerging infectious diseases Vol. 26; no. 7; pp. 1633 - 1635
• Main Authors: Waggoner, Jesse J., Stittleburg, Victoria, Pond, Renee, Saklawi, Youssef, Sahoo, Malaya K., Babiker, Ahmed, Hussaini, Laila, Kraft, Colleen S., Pinsky, Benjamin A., Anderson, Evan J., Rouphael, Nadine
• Format: Journal Article
• Language: English
• Published: United States U.S. National Center for Infectious Diseases 01.07.2020; Centers for Disease Control and Prevention
• Subjects: Betacoronavirus - genetics; Betacoronavirus - isolation & purification; Coronaviruses; COVID-19; Disease control; Disease prevention; Expedited; Genes; Humans; Infections; Medical laboratories; molecular detection; Multiplex Polymerase Chain Reaction - methods; Nasopharynx - virology; Pathogens; Real-Time Polymerase Chain Reaction - methods; Research Letter; respiratory infections; RNA; RNA polymerase; rRT-PCR; SARS-CoV-2; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Transcription (Genetics); Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2; Viral infections; Viruses; Atlanta Georgia; United States > US; Georgia; COVID-19; SARS-CoV-2; viruses; severe acute respiratory syndrome coronavirus 2; SARS; coronavirus; zoonoses; 2019 novel coronavirus disease; coronavirus disease; molecular detection; respiratory infections; rRT-PCR
• ISSN: 1080-6040; 1080-6059; 1080-6059
• DOI: 10.3201/eid2607.201285

Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.