Detecting SARS-CoV-2 Omicron B.1.1.529 Variant in Wastewater Samples by Using Nanopore Sequencing

We report wastewater surveillance for SARS-CoV-2 variants of concern by using mutation-specific, real-time PCR and rapid nanopore sequencing. This surveillance might be useful for an early warning in a scenario in which a new variant is emerging, even in areas that have low virus incidences.

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Bibliographic Details
Published inEmerging infectious diseases Vol. 28; no. 6; pp. 1296 - 1298
Main Authors Rasmussen, Lasse D, Richter, Stine R, Midgley, Sofie E, Franck, Kristina T
Format Journal Article
LanguageEnglish
Published United States U.S. National Center for Infectious Diseases 01.06.2022
Centers for Disease Control and Prevention
Subjects
Consortia
Coronaviruses
COVID-19
Detecting SARS-CoV-2 Omicron B.1.1.529 Variant in Wastewater Samples by Using Nanopore Sequencing
Disease transmission
DNA sequencing
Environmental aspects
Expedited
Genomes
Health aspects
Laboratories
Mutation
Nucleotide sequencing
Research Letter
respiratory infections
Risk factors
RNA polymerase
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
viruses
Wastewater
Denmark
nanopore sequencing
viruses
B.1.1.529
variants of concern
wastewater surveillance
zoonoses
coronaviruses
respiratory infections
COVID-19
SARS-CoV-2
emerging variants
severe acute respiratory syndrome coronavirus 2
Denmark. Omicron
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Summary:We report wastewater surveillance for SARS-CoV-2 variants of concern by using mutation-specific, real-time PCR and rapid nanopore sequencing. This surveillance might be useful for an early warning in a scenario in which a new variant is emerging, even in areas that have low virus incidences.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1080-6040
1080-6059
DOI:10.3201/eid2806.220194