Cloning and Expression of Calmodulin Gene in Scoparia dulcis

• Published in: Biological & Pharmaceutical Bulletin Vol. 30; no. 6; pp. 1161 - 1163
• Main Authors: Saitoh, Daisuke, Asakura, Yuki, Nkembo, Marguerite Kasidimoko, Shite, Masato, Sugiyama, Ryuji, Lee, Jung-Bum, Hayashi, Toshimitsu, Kurosaki, Fumiya
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Pharmaceutical Society of Japan 01.06.2007; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: Acetates - pharmacology; Amino Acid Sequence; Blotting, Southern; Calcimycin - pharmacology; calcium cascade; calmodulin; Calmodulin - chemistry; Calmodulin - genetics; Calmodulin - metabolism; Cells, Cultured; Cloning, Molecular; Cyclopentanes - pharmacology; diterpene biosynthesis; DNA, Complementary; gene cloning; Gene Expression; Ionophores - pharmacology; Molecular Sequence Data; Oxylipins; Plant Growth Regulators - pharmacology; Plant Leaves - cytology; Plant Leaves - genetics; Plant Roots - genetics; Plant Stems - genetics; Protein Structure, Tertiary; Reverse Transcriptase Polymerase Chain Reaction; Scoparia - cytology; Scoparia - genetics; Scoparia dulcis; Transcription, Genetic
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.30.1161

A homology-based cloning strategy yielded a cDNA clone, designated Sd-cam, encoding calmodulin protein from Scoparia dulcis. The restriction digests of genomic DNA of S. dulcis showed a single hybridized signal when probed with the fragment of this gene in Southern blot analyses, suggesting that Sd-cam occurs as a sole gene encoding calmodulin in the plant. The reverse-transcription polymerase chain reaction analysis revealed that Sd-cam was appreciably expressed in leaf, root and stem tissues. It appeared that transcription of this gene increased transiently when the leaf cultures of S. dulcis were treated with methyl jasmonate and calcium ionophore A23187. These results suggest that transcriptional activation of Sd-cam is one of the early cellular events of the methyl jasmonate-induced responses of S. dulcis.