Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography

In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve th...

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Bibliographic Details
Published inAnalytical Sciences Vol. 37; no. 5; pp. 651 - 660
Main Authors MAEKAWA, Yutaro, AYANO, Eri, NAGASE, Kenichi, KANAZAWA, Hideko
Format Journal Article
LanguageEnglish
Published Singapore The Japan Society for Analytical Chemistry 10.05.2021
Springer Nature Singapore
Japan Science and Technology Agency
Subjects
Analytical Chemistry
cell separation
Cell therapy
Chemistry
Chromatography
Chromatography, High Pressure Liquid
High performance liquid chromatography
Liquid chromatography
Monoclonal antibodies
monoclonal antibody
new therapeutic modality
Nucleic acids
oligonucleotide
poly(N-isopropylacrylamide)
Polyisopropyl acrylamide
Polymers
Proteins
Regenerative medicine
Reviews
Separation
smart functional surfaces
Solid Phase Extraction
Solid phases
Stationary phase
Temperature
Temperature-responsive chromatography
Temperature-responsive chromatography
isopropylacrylamide
poly
cell separation
monoclonal antibody
smart functional surfaces
new therapeutic modality
oligonucleotide
poly(N-isopropylacrylamide)
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Summary:In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, and regenerative medicine. Therefore, new approaches and technologies must be developed to respond to these changes in medical care. To achieve this, we applied a temperature-responsive separation system to purify a variety of proteins and cells. We developed a temperature-responsive chromatography technique based on a poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may be applied to various types of protein and cell separation applications by optimizing the properties of the modified polymers used in this system. Therefore, the developed temperature-responsive HPLC columns and temperature-responsive solid-phase extraction (TR-SPE) columns can be an effective separation tool for new therapeutic modalities such as monoclonal antibodies, nucleic acid drugs, and cells.
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.20SCR09