Measurement of 8-hydroxyguanine as an oxidative stress biomarker in saliva by HPLC-ECD

Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to col...

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Published inGenes and environment Vol. 40; no. 1; pp. 5 - 4
Main Authors Kawai, Kazuaki, Kasai, Hiroshi, Li, Yun-Shan, Kawasaki, Yuya, Watanabe, Shintaro, Ohta, Masanori, Honda, Toru, Yamato, Hiroshi
Format Journal Article
LanguageEnglish
Published England BioMed Central Ltd 04.04.2018
BioMed Central
BMC
Subjects
8-hydroxyguanine
Biomarker
High performance liquid chromatography
HPLC-ECD
Measurement
Oxidative stress
Saliva
Short Report
Oxidative stress
Biomarker
8-hydroxyguanine
HPLC-ECD
Saliva
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Summary:Oxidative stress leads to many kinds of diseases. Currently, urinary 8-hydroxydeoxyguanosine (8-OHdG) is widely measured as an oxidative stress biomarker. There is a specific advantage if saliva can be used as the sample to measure the oxidative stress biomarker, because saliva is much easier to collect than urine. In this study, we investigated the measurement of 8-hydroxyguanine (8-OHGua) as an oxidative stress marker in saliva, by a column switching HPLC system equipped with an electrochemical detector (HPLC-ECD). The 8-OHGua in saliva could be detected as a single peak by HPLC-ECD. The average level of 8-OHGua in saliva was 3.80 ng/mL in ordinary, non-smoking subjects. The salivary 8-OHGua levels of smokers were significantly higher than those of non-smokers. Salivary 8-OHGua may be a useful noninvasive and promising oxidative stress biomarker.
ISSN:1880-7046
1880-7062
1880-7062
DOI:10.1186/s41021-018-0095-2