Genetic transformation of a high molecular weight glutenin (Glu-1Dx5) to rice [Oryza sativa] cv. Fatmawati

• Published in: Plant production science Vol. 12; no. 3; pp. 341 - 344
• Main Authors: Wada, Y.(Utsunomiya Univ. (Japan)), Carsono, N, Anas, Tong, L, Yoshida, T
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 2009; Crop Science Society of Japan; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Agronomy. Soil science and plant productions; ARROZ; Biological and medical sciences; BLE TENDRE; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; GENETIC TRANSFORMATION; Glu-1Dx5; GLUTENINAS; GLUTENINE; GLUTENINS; High molecular weight glutenin; MOLECULAR WEIGHT; ORYZA SATIVA; PESO MOLECULAR; Plant extracts; POIDS MOLECULAIRE; RICE; RIZ; SOFT WHEAT; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Transgenic plants; TRIGO HARINERO; TRITICUM AESTIVUM; VARIEDADES; VARIETE; VARIETIES; Wheat; Plant production; Glu-1Dx5; Monocotyledones; Genetic transformation; High molecular weight glutenin; Oryza; High molecular weight; Gramineae; Angiospermae; Herbaceous plant; Spermatophyta; Glutenin; Rice
• ISSN: 1343-943X; 1349-1008
• DOI: 10.1626/pps.12.341

In order to improve rice dough functionality, we co-transformed the Glu-1Dx5 gene encoding a high molecular weight (HMW) glutenin subunit Dx5 from bread wheat, Triticum aestivum L. and either bar gene conferring resistance to herbicide bialaphos or hpt gene conferring resistance to hygromycin B to rice callus cells of cv. Fatmawati. We molecularly characterized 9 plants regenerated from bialaphos-containing medium and 63 plants from hygromycin-containing medium. The Glu-1Dx5 gene was detected by PCR analysis in 15 transgenic Tsub(0) Plants. Further analysis of Tsub(1) and Tsub(2) plants revealed that some transgenic plants carried the Glu-1Dx5 gene. Analysis of the endosperm extracts of Tsub(2) plants by SDS-PAGE revealed the existence of a protein similar in size to the wheat Glu-1Dx5 gene product, suggesting successful expression of the transgene. These plants will be incorporated into breeding program for further assessment of their benefits.