Integrated consensus genetic and physical maps of flax (Linum usitatissimum L.)

• Published in: Theoretical and applied genetics Vol. 125; no. 8; pp. 1783 - 1795
• Main Authors: Cloutier, Sylvie, Ragupathy, Raja, Miranda, Evelyn, Radovanovic, Natasa, Reimer, Elsa, Walichnowski, Andrzej, Ward, Kerry, Rowland, Gordon, Duguid, Scott, Banik, Mitali
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.12.2012; Springer; Springer Nature B.V
• Subjects: Agriculture; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome mapping; chromosome number; Contig Mapping; Corn; evolution; Flax; Flax - genetics; Flowers & plants; Genetic aspects; Genetic diversity; Genetic Linkage; Genetic Markers; genome; Genomes; Genomics; Germplasm; haploidy; Life Sciences; linkage groups; Linum usitatissimum; Oilseeds; Original Paper; Physical Chromosome Mapping - methods; Physiological aspects; Plant Biochemistry; Plant Breeding/Biotechnology; Plant genetics; Plant Genetics and Genomics; Polymorphism; Seeds; segregation distortion; Canada; China; Russia; Bacterial Artificial Chromosome Clone; Amplify Fragment Length Polymorphism; Bacterial Artificial Chromosome; Simple Sequence Repeat Marker; Double Haploid
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-012-1953-0

Three linkage maps of flax (Linum usitatissimum L.) were constructed from populations CDC Bethune/Macbeth, E1747/Viking and SP2047/UGG5-5 containing between 385 and 469 mapped markers each. The first consensus map of flax was constructed incorporating 770 markers based on 371 shared markers including 114 that were shared by all three populations and 257 shared between any two populations. The 15 linkage group map corresponds to the haploid number of chromosomes of this species. The marker order of the consensus map was largely collinear in all three individual maps but a few local inversions and marker rearrangements spanning short intervals were observed. Segregation distortion was present in all linkage groups which contained 1–52 markers displaying non-Mendelian segregation. The total length of the consensus genetic map is 1,551 cM with a mean marker density of 2.0 cM. A total of 670 markers were anchored to 204 of the 416 fingerprinted contigs of the physical map corresponding to ~274 Mb or 74 % of the estimated flax genome size of 370 Mb. This high resolution consensus map will be a resource for comparative genomics, genome organization, evolution studies and anchoring of the whole genome shotgun sequence.