Inhibitory Effects of Triterpenes Isolated from Chuling (Polyporus umbellatus FRIES) on Free Radical-Induced Lysis of Red Blood Cells

Chuling, sclerotia of Polyporus umbellatus FRIES, has long been used for urological disorders in traditional medicine. In this study, we demonstrated that Chuling in vitro protects red blood cells from 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was d...

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Bibliographic Details
Published inBiological & Pharmaceutical Bulletin Vol. 28; no. 5; pp. 817 - 821
Main Authors Sekiya, Nobuyasu, Hikiami, Hiroaki, Nakai, Yoichiro, Sakakibara, Iwao, Nozaki, Kazuya, Kouta, Kazufumi, Shimada, Yutaka, Terasawa, Katsutoshi
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 01.05.2005
Pharmaceutical Society of Japan
Japan Science and Technology Agency
Subjects
anti-oxidative activity
Chuling
Dose-Response Relationship, Drug
Erythrocytes - drug effects
Erythrocytes - metabolism
Free Radical Scavengers - isolation & purification
Free Radical Scavengers - pharmacology
Free Radicals - antagonists & inhibitors
Free Radicals - pharmacology
hemolysis
Hemolysis - drug effects
Hemolysis - physiology
Humans
Male
Polyporales - isolation & purification
Polyporus umbellatus
triterpene
Triterpenes - isolation & purification
Triterpenes - pharmacology
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Summary:Chuling, sclerotia of Polyporus umbellatus FRIES, has long been used for urological disorders in traditional medicine. In this study, we demonstrated that Chuling in vitro protects red blood cells from 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 50 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of Chuling with scavenging effect on free radicals. Triterpene carboxylic acids isolated from the methanol extract of Chuling, namely, polyporusterone A and polyporusterone B, were found to have inhibitory activities against AAPH-induced lysis of red blood cells. The anti-hemolytic effect was significantly stronger in polyporusterone B compared with polyporusterone A. Furthermore, the ingestion of 150 mg of Chuling was associated with a significant increase in free-radical scavenging effect of plasma in rats.
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ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.28.817