Expression and Regulation of Periostin/OSF-2 Gene in Rat Uterus and Human Endometrium

• Published in: ENDOCRINE JOURNAL Vol. 55; no. 1; pp. 183 - 189
• Main Authors: HIROI, Hisahiko, MOMOEDA, Mikio, NAKAZAWA, Fumiko, KOIZUMI, Minako, TSUTSUMI, Ryo, HOSOKAWA, Yumi, OSUGA, Yutaka, YANO, Tetsu, TSUTSUMI, Osamu, TAKETANI, Yuji
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japan Endocrine Society 2008
• Subjects: Animals; Cell Adhesion Molecules - genetics; Cell Adhesion Molecules - metabolism; Cell Culture Techniques; Cells, Cultured; Endometrium - metabolism; Estrogen; Female; Gene Expression Regulation - drug effects; Human endometiurm; Humans; Menstrual Cycle - drug effects; Menstrual Cycle - genetics; Menstrual Cycle - metabolism; Periostin/OSF-2; Progesterone - pharmacology; Rat uterus; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism; Stromal Cells - metabolism; Time Factors; Tissue Distribution; Uterus - metabolism
• ISSN: 0918-8959; 1348-4540
• DOI: 10.1507/endocrj.K07-073

Periostin/OSF2 is a ligand for αvβ3 and αvβ5 integrins and activates the Akt/PKB pathway. Recent reports of periostin/OSF2 gene disrupted mice indicate that periostin/OSF-2 plays an important role in implantation. Quantitative RT-PCR revealed that the expression of periostin/OSF-2 mRNA in rat uteri was reduced to approximately 10% at 12 h after 17β-estradiol (E2) injection, but was not changed after progesterone (P) injection. RT-PCR revealed the expression of periostin/OSF-2 in human endometrium, cultured human endometrial stromal cells (ESCs), and cultured human endometrial epithelial cells. In ESCs, the expression of periostin/OSF-2 mRNA was reduced to approximately 50% at 6 h after E2 treatment. The amount of periostin/OSF2 mRNA in human endometrium significantly increased during mid-proliferative and early secretory phases of menstrual cycle, and decreased during late-proliferative, mid-secretory and late secretory phases. The expression of periostin/OSF2 mRNA significantly decreased in ESCs decidualized by treatment with E2 and P for 7 and 11 days. By immunohistochemistry, the expression of periostin/OSF-2 was strongly detected in endometrial stromal cells during early proliferative, mid-proliferative and early secretory phases, and was strongly detected in endometrial epithelial cells during late secretory phase. This study demonstrated that the expression of periostin/OSF-2 is regulated by ovarian steroid hormones in rat uterus and human endometrium.