Puerarin protects against damage to spatial learning and memory ability in mice with chronic alcohol poisoning

• Published in: Brazilian journal of medical and biological research Vol. 48; no. 6; pp. 515 - 522
• Main Authors: Cui, S Q, Wang, Q, Zheng, Y, Xiao, B, Sun, H W, Gu, X L, Zhang, Y C, Fu, C H, Dong, P X, Wang, X M
• Format: Journal Article
• Language: English
• Published: Brazil Associacao Brasileira de Divulgacao Cientifica (ABDC) 01.06.2015; Associação Brasileira de Divulgação Científica
• Subjects: Alcohol; Alcoholism - complications; Animals; BIOLOGY; Biomedical Sciences; Cerebral Cortex - chemistry; Cerebral Cortex - drug effects; Chromatography, High Pressure Liquid; Chronic alcohol poisoning; Enzyme-Linked Immunosorbent Assay; Ethanol - poisoning; gamma-Aminobutyric Acid - analysis; Glutamic Acid - analysis; Health aspects; Inflammatory factors; Interleukin-1beta - analysis; Isoflavones; Isoflavones - pharmacology; Isoflavones - therapeutic use; Male; Maze Learning - drug effects; MEDICINE, RESEARCH & EXPERIMENTAL; Memory Disorders - chemically induced; Memory Disorders - drug therapy; Memory Disorders - prevention & control; Memory, Disorders of; Mice, Inbred C57BL; Microglia; Microglia - drug effects; Neuroprotection; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; Prevention; Puerarin; Random Allocation; Risk factors; Spatial Memory - drug effects; Time Factors; Treatment Outcome; Tumor Necrosis Factor-alpha - analysis; Vasodilator Agents - pharmacology; Vasodilator Agents - therapeutic use; China; Inflammatory factors; Neuroprotection; Chronic alcohol poisoning; Puerarin; Microglia
• ISSN: 0100-879X; 1414-431X; 1414-431X
• DOI: 10.1590/1414-431X20144250

We evaluated the effect of puerarin on spatial learning and memory ability of mice with chronic alcohol poisoning. A total of 30 male C57BL/6 mice were randomly divided into model, puerarin, and control groups (n=10 each). The model group received 60% (v/v) ethanol by intragastric administration followed by intraperitoneal injection of normal saline 30 min later. The puerarin group received intragastric 60% ethanol followed by intraperitoneal puerarin 30 min later, and the control group received intragastric saline followed by intraperitoneal saline. Six weeks after treatment, the Morris water maze and Tru Scan behavioral tests and immunofluorescence staining of cerebral cortex and hippocampal neurons (by Neu-N) and microglia (by Ib1) were conducted. Glutamic acid (Glu) and gamma amino butyric acid (GABA) in the cortex and hippocampus were assayed by high-performance liquid chromatography (HPLC), and tumor necrosis factor (TNF)-α and interleukin (IL)-1β were determined by ELISA. Compared with mice in the control group, escape latency and distance were prolonged, and spontaneous movement distance was shortened (P<0.05) by puerarin. The number of microglia was increased in both the cortex and hippocampal dentate gyrus (P<0.01), and neurons were reduced only in the hippocampal dentate gyrus (P<0.01) in puerarin-treated mice. In the model group, Glu and GABA levels decreased (P<0.05), and Glu/GABA, TNF-α, and IL-1β increased (P<0.01) with puerarin treatment, returning to near normal levels. In conclusion, puerarin protected against the effects of chronic alcohol poisoning on spatial learning and memory ability primarily because of anti-inflammatory activity and regulation of the balance of Glu and GABA.