The duck genome and transcriptome provide insight into an avian influenza virus reservoir species
Ning Li and colleagues report the whole-genome sequence of the duck, Anas platyrhynchos , a natural host of avian influenza viruses. They examine host response to infection by comparing the lung transcriptomes of ducks that were infected with influenza A viruses. The duck ( Anas platyrhynchos ) is o...
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Published in | Nature genetics Vol. 45; no. 7; pp. 776 - 783 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.07.2013
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Ning Li and colleagues report the whole-genome sequence of the duck,
Anas platyrhynchos
, a natural host of avian influenza viruses. They examine host response to infection by comparing the lung transcriptomes of ducks that were infected with influenza A viruses.
The duck (
Anas platyrhynchos
) is one of the principal natural hosts of influenza A viruses. We present the duck genome sequence and perform deep transcriptome analyses to investigate immune-related genes. Our data indicate that the duck possesses a contractive immune gene repertoire, as in chicken and zebra finch, and this repertoire has been shaped through lineage-specific duplications. We identify genes that are responsive to influenza A viruses using the lung transcriptomes of control ducks and ones that were infected with either a highly pathogenic (A/duck/Hubei/49/05) or a weakly pathogenic (A/goose/Hubei/65/05) H5N1 virus. Further, we show how the duck's defense mechanisms against influenza infection have been optimized through the diversification of its β-defensin and butyrophilin-like repertoires. These analyses, in combination with the genomic and transcriptomic data, provide a resource for characterizing the interaction between host and influenza viruses. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 PMCID: PMC4003391 AUTHOR CONTRIBUTIONS N.L., J.W., Y.H. and D.W.B. organized the committee for the duck genome sequencing project. N.L., J.W., Y.H., X.H., L.R. and J.F. designed the duck genome sequence project. J.W., W.Q., Y.L. and Y. Zhang sequenced and assembled the duck genome. W.Q., Y.H., A.V. and T.F. assessed the quality of the duck genome. J.L., W.Q., S.F., Y.H., B.L., A.V., S.S., Yiqiang Zhao, Z.D., Q.C., H.T., S.B., S.K., M. Marz, M. Morisson, M.R., F.P. and P.F.S. performed gene prediction and annotation. D.W.B., H.K., Y.H., B.L., J.H., T.L., K.-W.K., J.S. and D.K.G. performed gene evolutionary analysis. Y.H., W.Q., D.W.B., Q.L., Z.D., Z.S., Y.A. and P.K. detected expansion and contraction of immune-related genes. Y.H., N.L., S.G., W.Q., Z.S. and Y.A. analyzed β-defensin and immunoglobulin genes. Y.H., N.L., H.C., K.Y., H.F., P.Z., D.W.B., K.E.M., R.G.W., J.R.A. and W.C.W. characterized the immune-related gene response to avian influenza viruses. Y.H. and W.Q. wrote the manuscript. N.L., Y.H., D.W.B., L.G., J.W., M.A.M.G., R.P.M.A.C., Yaofeng Zhao, R.H.S.K., A.V., K.E.M. and J.S. revised the manuscript. |
ISSN: | 1061-4036 1546-1718 |
DOI: | 10.1038/ng.2657 |