Types of Parvalbumin-Containing Retinotectal Ganglion Cells in Mouse
The calcium-binding protein parvalbumin (PV) occurs in the retinal ganglion cells (RGCs) of various vertebrate species. In the present study, we aimed to identify the types of PV-containing RGCs that project to the superior colliculus (SC) in the mouse. We injected retrograde tracer dextran into the...
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Published in | ACTA HISTOCHEMICA ET CYTOCHEMICA Vol. 45; no. 3; pp. 201 - 210 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
01.01.2012
Japan Science and Technology Agency Japan Society of Histochemistry and Cytochemistry |
Subjects | |
Online Access | Get full text |
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Summary: | The calcium-binding protein parvalbumin (PV) occurs in the retinal ganglion cells (RGCs) of various vertebrate species. In the present study, we aimed to identify the types of PV-containing RGCs that project to the superior colliculus (SC) in the mouse. We injected retrograde tracer dextran into the mouse SC to label RGCs. PV-containing RGCs were first identified by immunocytochemistry and then neurons double-labeled with dextran and PV were iontophoretically injected with a lipophilic dye, DiI. Subsequently, confocal microscopy was used to characterize the morphologic classification of the PV-immunoreactive (IR) retinotectal ganglion cells on the basis of dendritic field size, branching pattern, and stratification within the inner plexiform layer. Among the 8 different types of PV-containing RGCs in the mouse retina, we found all 8 types of RGCs projecting to the SC. The RGCs were heterogeneous in morphology. The combined approach of using tracer injection and a single cell injection after immunocytochemistry on a particular protein will provide valuable data to further understand the functional features of the RGCs which constitute the retinotectal pathway. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0044-5991 1347-5800 |
DOI: | 10.1267/ahc.11061 |