A serological assay to detect SARS-CoV-2 seroconversion in humans

• Published in: Nature medicine Vol. 26; no. 7; pp. 1033 - 1036
• Main Authors: Amanat, Fatima, Stadlbauer, Daniel, Strohmeier, Shirin, Nguyen, Thi H. O., Chromikova, Veronika, McMahon, Meagan, Jiang, Kaijun, Arunkumar, Guha Asthagiri, Jurczyszak, Denise, Polanco, Jose, Bermudez-Gonzalez, Maria, Kleiner, Giulio, Aydillo, Teresa, Miorin, Lisa, Fierer, Daniel S., Lugo, Luz Amarilis, Kojic, Erna Milunka, Stoever, Jonathan, Liu, Sean T. H., Cunningham-Rundles, Charlotte, Felgner, Philip L., Moran, Thomas, García-Sastre, Adolfo, Caplivski, Daniel, Cheng, Allen C., Kedzierska, Katherine, Vapalahti, Olli, Hepojoki, Jussi M., Simon, Viviana, Krammer, Florian
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.07.2020; Nature Publishing Group
• Subjects: 631/250/2152; 631/326/590; 631/326/596; Adult; Analysis; Antibodies; Antigens; Assaying; Betacoronavirus - immunology; Betacoronavirus - pathogenicity; Biomedical and Life Sciences; Biomedicine; Brief Communication; Cancer Research; Case-Control Studies; Clinical Laboratory Techniques - methods; Coronavirus Infections - blood; Coronavirus Infections - diagnosis; Coronavirus Infections - immunology; Coronavirus Infections - therapy; Coronavirus Infections - virology; Coronaviruses; COVID-19; COVID-19 Testing; Diagnosis; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Enzymes; Health aspects; Health care; Humans; Identification and classification; Immunization, Passive; Immunology; Infections; Infectious disease incubation period; Infectious Diseases; Longitudinal Studies; Metabolic Diseases; Methods; Middle Aged; Molecular Medicine; Neurosciences; Neutralization Tests; Pandemics; Physiological aspects; Plasma; Pneumonia, Viral - diagnosis; Pneumonia, Viral - immunology; Pneumonia, Viral - therapy; Pneumonia, Viral - virology; Preventive medicine; Proteins; SARS-CoV-2; Seroconversion; Serodiagnosis; Serology; Severe acute respiratory syndrome coronavirus 2; Spike protein; Viral diseases; Viral infections; Virology; Viruses; Young Adult; United States
• ISSN: 1078-8956; 1546-170X; 1546-170X
• DOI: 10.1038/s41591-020-0913-5

Here, we describe a serological enzyme-linked immunosorbent assay for the screening and identification of human SARS-CoV-2 seroconverters. This assay does not require the handling of infectious virus, can be adjusted to detect different antibody types in serum and plasma and is amenable to scaling. Serological assays are of critical importance to help define previous exposure to SARS-CoV-2 in populations, identify highly reactive human donors for convalescent plasma therapy and investigate correlates of protection. Development of an enzyme-linked immunosorbent assay to detect antibodies to the SARS-CoV-2 spike protein in human sera and plasma.