Development of a Microfluidic System Comprising Dialysis and Secretion Components for a Bioassay of Renal Clearance

• Published in: Analytical Sciences Vol. 34; no. 9; pp. 1073 - 1078
• Main Authors: SAKUTA, Yu, TAKEHARA, Issey, TSUNODA, Kin-ichi, SATO, Kiichi
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 10.09.2018; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Active transport; Analytical Chemistry; Bioassays; Chemistry; Dialysis; Excretion; Glycoproteins; Hemodialysis; Kidney-on-a-chip; Kidneys; microchip; microfluidic device; Microfluidics; organ-on-a-chip; P-Glycoprotein; Quinidine; renal excretion; Rhodamine; Secretion; Size separation; Substrates; Sulfates; Transport; tubular secretion; renal excretion; Kidney-on-a-chip; microfluidic device; tubular secretion; organ-on-a-chip; glycoprotein; microchip; P-glycoprotein
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.18P141

We have developed a microfluidic bioassay system that mimics glomerular filtration and tubular secretion in the kidney. The system consists of a peristaltic micropump (heart), a dialysis component (renal corpuscle), and a secretion component (renal proximal tubule). Analytes were separated by size using a dialysis membrane in the dialysis component. Model cells were cultured on a membrane in the secretion component, and active transport mediated by P-glycoprotein (P-gp) was confirmed using the P-gp substrate rhodamine 123 with or without the P-gp inhibitor quinidine sulfate. The system achieved both size separation and selective transport by P-gp on a single microchip. This proof-of-concept model may find applications in drug excretion assays, including studies of drug-drug interactions during tubular secretion.