Diversity of the bacteria in the roots of sugarcane used to produce Wasanbon in Kagawa, Japan

• Published in: Plant Biotechnology Vol. 37; no. 1; pp. 99 - 103
• Main Authors: Supriadi, Shimoura, Natsuki, Miyaji, Masahiro, Tajima, Shigeyuki, Nomura, Mika
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society for Plant Biotechnology 25.03.2020; Japan Science and Technology Agency; Japanese Society for Plant Cell and Molecular Biology
• Subjects: 16S rRNA; Bacteria; Deoxyribonucleic acid; DNA; endophyte; Endophytes; NifH gene; Nitrogen fixation; Nitrogen-fixing bacteria; Nitrogenation; Nutrient cycles; Nutrients; Plant growth; Plant tissues; Roots; rRNA 16S; Saccharum sinense; Soil bacteria; Soil microorganisms; Soils; Sugarcane; Wasanbon; Japan; 16S rRNA; Wasanbon; endophyte; sugarcane
• ISSN: 1342-4580; 1347-6114
• DOI: 10.5511/plantbiotechnology.20.0107a

Soil bacteria are an important factor in the cycle of nutrients in soil, while root bacteria in the internal tissues of plants can promote plant growth. The aim of this research was to study the diversity of root bacteria of sugarcane grown in Kagawa, Japan. The sugarcane, derived from Saccharum sinense and grown only in this area, is used as a raw material for Wasanbon (a fine-grained Japanese sugar) and is characterized by thin stalks and a low sugar content. To determine its bacterial diversity, bacterial DNA was extracted from the soil and roots, and 16S rRNA genes were sequenced. A total of 1259 operational taxonomic units (OTUs) were detected in the root bacteria and 3894 OTUs in the soil bacteria. The α-diversity between the soil and root bacteria was significantly different. The most abundant class of root and soil bacteria was proteobacteria at more than 50 and 30%, respectively. The endophyte bacteria of rhizobium were also isolated and the nifH gene was detected. The relationship between the application of nitrogen-fixing bacteria and the production of Wasanbon should be studied.