Protection of Testicular Dysfunctions by MTEC, a Formulated Herbal Drug, in Streptozotocin Induced Diabetic Rat

• Published in: Biological & Pharmaceutical Bulletin Vol. 30; no. 1; pp. 84 - 90
• Main Authors: Mallick, Chhanda, Mandal, Suvra, Barik, Bikashranjan, Bhattacharya, Atanu, Ghosh, Debidas
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Pharmaceutical Society of Japan 2007; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: 17-Hydroxysteroid Dehydrogenases - metabolism; 3-Hydroxysteroid Dehydrogenases - metabolism; androgenic key enzyme; Animals; Blood Glucose - drug effects; Body Weight - drug effects; Catalase - metabolism; Cell Survival - drug effects; diabetes; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - drug therapy; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Glucose - metabolism; herbal drug; Hypoglycemic Agents - pharmacology; Hypoglycemic Agents - therapeutic use; insulin; Insulin - blood; Lipid Peroxidation - drug effects; Male; oxidative stress; Peroxidase - metabolism; Rats; Rats, Wistar; Sperm Count; Sperm Motility - drug effects; Spermatozoa - drug effects; Testicular Diseases - blood; Testicular Diseases - etiology; Testicular Diseases - pathology; Testicular Diseases - physiopathology; Testicular Diseases - prevention & control; Testis - drug effects; Testis - metabolism; Testis - pathology; Testis - physiopathology; testosterone; Testosterone - blood; Time Factors
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.30.84

Single injection of streptozotocin (STZ) resulted diabetes mellitus which was reflected here by the levels of fasting blood glucose and serum insulin. Moreover, this experimental diabetes also resulted testicular dysfunctions evaluated by count, viability and motility of sperm as well as by the activities of key enzymes for androgen synthesis. Diabetes induced testicular oxidative stress has been indicated here by the monitoring of testicular peroxidase and catalase activities as well as by quantification of TBARS and CD of testis. Testicular glucose was increased and leydig cell nuclear area was decreased in STZ induced diabetes. Treatment of herbal formulated drug named as MTEC consist of aqueous-methanol extract of Musa paradisiaca, Tamarindus indica, Eugenia jambolana and Coccinia indica to streptozotocin induced diabetic rat at the ratio of 2 : 2 : 1 : 1 at the dose of 60 mg/d for two times a day for 14 d resulted a significant protection in fasting blood glucose and serum insulin levels (p<0.05) along with correction of testicular above parameters towards the control level (p<0.05). This herbal formulated drug has no general toxic effects on the body weight, as well as on the activities of serum glutamate and pyruvate transaminases in serum. The results support the validity of this herbal drug for the management of testicular disorders noted in diabetic state.