Hyperglycemia-Induced Reactive Oxygen Species Toxicity to Endothelial Cells Is Dependent on Paracrine Mediators

• Published in: Diabetes (New York, N.Y.) Vol. 57; no. 7; pp. 1952 - 1965
• Main Authors: Busik, Julia V., Mohr, Susanne, Grant, Maria B.
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Diabetes Association 01.07.2008
• Subjects: Annexin A5 - metabolism; Biological and medical sciences; Caspases - metabolism; Cell culture; Cell Culture Techniques; Cell death; Chronic illnesses; Complications; Cytokines; Cytokines - pharmacology; Development and progression; Diabetes; Diabetes. Impaired glucose tolerance; Diabetic retinopathy; Diabetic Retinopathy - etiology; Diabetic Retinopathy - physiopathology; Electromagnetic Phenomena; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Glucose; Glucose - metabolism; Humans; Hyperglycemia; Hyperglycemia - complications; Hyperglycemia - physiopathology; Kinases; Medical sciences; Mitochondria; Mitochondria - metabolism; Oxidative Stress; Phosphorylation; Physiological aspects; Pigment Epithelium of Eye - cytology; Pigment Epithelium of Eye - drug effects; Pigment Epithelium of Eye - metabolism; Proteins; Reactive Oxygen Species - metabolism; Reactive Oxygen Species - toxicity; Research design; Risk factors; Superoxides - metabolism; Toxicity; Tumor necrosis factor-TNF; Vascular Cell Adhesion Molecule-1 - genetics; Canada; Endocrinopathy; Endothelial cell; Hyperglycemia; Oxygen; Toxicity; Diabetes mellitus
• ISSN: 0012-1797; 1939-327X; 1939-327X
• DOI: 10.2337/db07-1520

Hyperglycemia-Induced Reactive Oxygen Species Toxicity to Endothelial Cells Is Dependent on Paracrine Mediators Julia V. Busik 1 , Susanne Mohr 2 and Maria B. Grant 3 1 Department of Physiology, Michigan State University, East Lansing, Michigan 2 Department of Medicine, Case Western Reserve University, Cleveland, Ohio 3 Department of Pharmacology and Therapeutics, University of Florida, Gainesville, Florida Corresponding author: Maria B. Grant, grantma{at}ufl.edu Abstract OBJECTIVE— This study determined the effects of high glucose exposure and cytokine treatment on generation of reactive oxygen species (ROS) and activation of inflammatory and apoptotic pathways in human retinal endothelial cells (HRECs). RESEARCH DESIGN AND METHODS— Glucose consumption of HRECs, human retinal pigment epithelial cells (HRPEs), and human Müller cells (HMCs) under elevated glucose conditions was measured and compared with cytokine treatment. Production of ROS in HRECs was examined using 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate (CM-H 2 DCFDA), spin-trap electron paramagnetic resonance, and MitoTracker Red staining after high glucose and cytokine treatment. The activation of different signaling cascades, including the mitogen-activated protein kinase pathways, tyrosine phosphorylation pathways, and apoptosis by high glucose and cytokines in HRECs, was determined. RESULTS— HRECs, in contrast to HRPEs and HMCs, did not increase glucose consumption in response to increasing glucose concentrations. Exposure of HRECs to 25 mmol/l glucose did not stimulate endogenous ROS production, activation of nuclear factor-κB (NF-κB), extracellular signal–related kinase (ERK), p38 and Jun NH 2 -terminal kinase (JNK), tyrosine phosphorylation, interleukin (IL)-1β, or tumor necrosis factor-α (TNF-α) production and only slightly affected apoptotic cell death pathways compared with normal glucose (5 mmol/l). In marked contrast, exposure of HRECs to proinflammatory cytokines IL-1β or TNF-α increased glucose consumption, mitochondrial superoxide production, ERK and JNK phosphorylation, tyrosine phosphorylation, NF-κB activation, and caspase activation. CONCLUSIONS— Our in vitro results indicate that HRECs respond to cytokines rather than high glucose, suggesting that in vivo diabetes–related endothelial injury in the retina may be due to glucose-induced cytokine release by other retinal cells and not a direct effect of high glucose. Footnotes Published ahead of print at http://diabetes.diabetesjournals.org on 16 April 2008. J.V.B. and S.M. contributed equally to this study. Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. Accepted April 9, 2008. Received October 26, 2007. DIABETES