Construction of Genomic Library and High-Throughput Screening of Pseudomonas aeruginosa Novel Antigens for Potential Vaccines

• Published in: Biological & pharmaceutical bulletin Vol. 43; no. 10; pp. 1469 - 1475
• Main Authors: Xu, Wanting, Li, Lei, Wen, Xiaobin, Liu, Qun, Liu, Yan, Wang, Xingyong, Lei, Langhuan, Chen, Qiushan, Liu, Li
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.10.2020; Pharmaceutical Society of Japan; Japan Science and Technology Agency
• Subjects: antigen screening; Antigens; Drug resistance; genome-wide library; Genomes; Genomics; High-throughput screening; Immunocompromised hosts; Immunoreactivity; Multidrug resistance; Pseudomonas aeruginosa; Recombinants; Vaccines
• ISSN: 0918-6158; 1347-5215; 1347-5215
• DOI: 10.1248/bpb.b19-01052

Hospital-acquired infections with Pseudomonas aeruginosa have become a great challenge in caring for critically ill and immunocompromised patients. The cause of high mortality is the presence of multi-drug resistant (MDR) strains, which confers a pressing need for vaccines. Although vaccines against P. aeruginosa have been in development for more than several decades, there is no vaccine for patients at present. In this study, we purified genomic DNA of P. aeruginosa from sera of patients affected, constructed genome-wide library with random recombinants, and screened candidate protein antigens by evaluating their protective effects in vivo. After 13-round of screening, 115 reactive recombinants were obtained, among which 13 antigens showed strong immunoreactivity (more than 10% reaction to PcrV, a well-characterized V-antigen of P. aeruginosa). These 13 antigens were: PpiA, PtsP, OprP, CAZ10_34235, HmuU_2, PcaK, CarAd, RecG, YjiR_5, LigD, KinB, RtcA, and PscF. In vivo studies showed that vaccination with PscF protected against lethal P. aeruginosa challenge, and decreased lung inflammation and injury. A genomic library of P. aeruginosa could be constructed in this way for the first time, which could not only screen candidate antigens but also in a high-throughput way. PscF was considered as an ideal promising vaccine candidate for combating P. aeruginosa infection and was supported for further evaluation of its safety and efficacy.