Decreased Anti-Müllerian hormone and Anti-Müllerian hormone receptor type 2 in hypothalami of old Japanese Black cows

• Published in: Journal of Veterinary Medical Science Vol. 82; no. 8; pp. 1113 - 1117
• Main Authors: KEREILWE, Onalenna, KADOKAWA, Hiroya
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2020; The Japanese Society of Veterinary Science
• Subjects: Age Factors; Animals; Anti-Mullerian Hormone - metabolism; Cattle; Female; female reproductive senescence; gonadotropin-releasing hormone neuron; Hypothalamus - metabolism; Müllerian inhibiting substance; preoptic area; Receptors, Peptide - genetics; Receptors, Peptide - metabolism; Receptors, Transforming Growth Factor beta - genetics; Receptors, Transforming Growth Factor beta - metabolism; ruminant; Theriogenology; ruminant; gonadotropin-releasing hormone neuron; Müllerian inhibiting substance; female reproductive senescence; preoptic area
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.20-0159

Cow fertility decreases with age, but the hypothalamic pathomechanisms are not understood. Anti-Müllerian hormone (AMH) stimulates gonadotropin-releasing hormone (GnRH) neurons via AMH receptor type 2 (AMHR2), and most GnRH neurons in the preoptic area (POA), arcuate nucleus (ARC), and median eminence (ME) express AMH and AMHR2. Therefore, we hypothesized that both protein amounts would differ in the anterior hypothalamus (containing the POA) and posterior hypothalamus (containing the ARC and ME) between young post-pubertal heifers and old cows. Western blot analysis showed lower (P<0.05) expressions of AMH and AMHR2 in the posterior hypothalamus, but not in the anterior hypothalamus, of old Japanese Black cows compared to young heifers. Therefore, AMH and AMHR2 were decreased in the posterior hypothalami of old cows.