Curcumin upregulates S100 expression and improves regeneration of the sciatic nerve following its complete amputation in mice

• Published in: Neural regeneration research Vol. 11; no. 8; pp. 1304 - 1311
• Main Authors: Liu, Guo-min, Xu, Kun, Li, Juan, Luo, Yun-gang
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer - Medknow Publications 01.08.2016; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Department of 0rthopedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China%Department of Health Laboratory, School of Public Health, Jilin University, Changchun, Jilin Province, China%Department of Stomatology, the Second Hospital of Jilin University, Changchun, Jilin Province, China; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Amputation; Analysis; Apoptosis; balb/c小鼠; Binding sites; Bioengineering; Curcumin; Cybernetics; Drug dosages; Gene expression; Laboratory animals; Nerve regeneration; nerve regeneration; electrophysiology; complete nerve amputation; spinal cord; myelin sheath; myelinated fiber; epineurium anatomosis; neural regeneration; Proteins; S100蛋白; Sciatic nerve; Spinal cord; 周围神经损伤; 坐骨神经; 姜黄素; 截肢; 神经传导速度; 神经再生; United States > US; China; epineurium anatomosis; neural regeneration; electrophysiology; myelinated fiber; myelin sheath; nerve regeneration; spinal cord; complete nerve amputation
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.189196

The repair of peripheral nerve injury after complete amputation is difficult,and even with anastomosis,the rapid recovery of nerve function remains challenging.Curcumin,extracted from plants of the genus Curcuma,has been shown to have anti-oxidant and anti-inflammatory properties and to improve sciatic nerve crush injury in rats.Here,we determined whether curcumin had neuroprotective effects following complete peripheral nerve amputation injury.BALB/c mice underwent complete sciatic nerve amputation,followed by an immediate epineurium anastomosis.Mice were intragastrically administered curcumin at doses of 40（high）,20（moderate）,and 10 mg/kg/d（low） for 1 week.We found that myelin in the mice of the high- and moderate-dose curcumin groups appeared with regular shape,uniform thickness,clear boundary,and little hyperplasia surrounding the myelin.High and moderate doses of curcumin markedly improved both action potential amplitude of the sciatic nerves and the conduction velocity of the corresponding motor neurons,and upregulated m RNA and protein expression of S100,a marker for Schwann cell proliferation,in L4–6 spinal cord segments.These results suggest that curcumin is effective in promoting the repair of complete sciatic nerve amputation injury and that the underlying mechanism may be associated with upregulation of S100 expression.