Moxibustion upregulates hippocampal progranulin expression

• Published in: Neural regeneration research Vol. 11; no. 4; pp. 610 - 616
• Main Authors: Yi, Tao, Qi, Li, Li, Ji, Le, Jing-jing, Shao, Lei, Du, Xin, Dong, Jing-cheng
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer - Medknow Publications 01.04.2016; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, China%E-institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Analysis; Animal cognition; Behavior; Chronic fatigue syndrome; Gene expression; Health aspects; Laboratory animals; Moxibustion; nerve regeneration; traditional Chinese medicine; moxibustion; chronic fatigue syndrome; hypothalamic-pituitary-adrenal axis; corticotrophin-releasing hormone; adrenocorticotropic hormone; behavioral symptoms; corticosterone; hippocampus; progranulin; neural regeneration; Rodents; Swimming; 下丘脑-垂体-肾上腺轴; 促肾上腺皮质激素释放激素; 前体; 海马; 艾灸; 蛋白; 酶联免疫吸附测定法; 颗粒; United States > US; China; corticotrophin-releasing hormone; hypothalamic-pituitary-adrenal axis; neural regeneration; hippocampus; chronic fatigue syndrome; progranulin; traditional Chinese medicine; nerve regeneration; corticosterone; adrenocorticotropic hormone; behavioral symptoms; moxibustion
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.180746

In China,moxibustion is reported to be useful and has few side effects for chronic fatigue syndrome,but its mechanisms are largely unknown.More recently,the focus has been on the wealth of information supporting stress as a factor in chronic fatigue syndrome,and largely concerns dysregulation in the stress-related hypothalamic-pituitary-adrenal axis.In the present study,we aimed to determine the effect of moxibustion on behavioral symptoms in chronic fatigue syndrome rats and examine possible mechanisms.Rats were subjected to a combination of chronic restraint stress and forced swimming to induce chronic fatigue syndrome.The acupoints Guanyuan（CV4） and Zusanli（ST36,bilateral） were simultaneously administered moxibustion.Untreated chronic fatigue syndrome rats and normal rats were used as controls.Results from the forced swimming test,open field test,tail suspension test,real-time PCR,enzyme-linked immunosorbent assay,and western blot assay showed that moxibustion treatment decreased m RNA expression of corticotropin-releasing hormone in the hypothalamus,and adrenocorticotropic hormone and corticosterone levels in plasma,and markedly increased progranulin m RNA and protein expression in the hippocampus.These findings suggest that moxibustion may relieve the behavioral symptoms of chronic fatigue syndrome,at least in part,by modulating the hypothalamic-pituitary-adrenal axis and upregulating hippocampal progranulin.