Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces

• Published in: International journal of nanomedicine Vol. 9; no. Issue 1; pp. 2753 - 2765
• Main Authors: Yang, Xi, Zhu, Liping, Tada, Seiichi, Zhou, Di, Kitajima, Takashi, Isoshima, Takashi, Yoshida, Yasuhiro, Nakamura, Mariko, Yan, Weiqun, Ito, Yoshihiro
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2014; Taylor & Francis Ltd; Dove Press; Dove Medical Press
• Subjects: Adhesives; Adhesives - chemical synthesis; Adhesives - pharmacology; Animals; Bioengineering; Biomedical materials; Biomimetic Materials - chemistry; Bivalvia - chemistry; Cell adhesion; Cell Adhesion - drug effects; Cell Adhesion - physiology; cell culture; Cells, Cultured; Collagen; Contact angle; Dopamine; Dopamine - chemistry; Dopamine - pharmacology; Endothelial Cells - drug effects; Endothelial Cells - physiology; Gelatin; Gelatin - chemistry; Gelatin - pharmacology; Humans; Materials Testing; natural catechols; Original Research; Physiological aspects; Proteins; recombinant human gelatin; Tissue engineering; Titanium; Vascular endothelial growth factor; Japan; natural catechols; cell culture; dopamine; cell adhesion; recombinant human gelatin; titanium
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/ijn.s60624

Recombinant human gelatin was conjugated with dopamine using carbodiimide as a surface modifier. This dopamine-coupled human gelatin (D-rhG) was characterized by (1)H-nuclear magnetic resonance, mass spectroscopy, and circular dichroism. D-rhG-coated surface properties were analyzed by physicochemical methods. Additionally, cell attachment and growth on the modified surfaces was assessed using human umbilical endothelial cells. Binding of gelatin onto titanium was significantly enhanced by dopamine conjugation. The thickness of the D-rhG coating depended on the treatment pH; thicker layers were formed at higher pH values, with a maximum thickness of 30 nm. D-rhG enhanced the binding of collagen-binding vascular endothelial growth factor and cell adhesion as compared with gelatin alone, even at the same surface concentration. The D-rhG surface modifier enhanced substrate binding by creating an adhesive nanointerface that increased specific protein binding and cell attachment.