Repair of patellar tendon injuries using a cell–collagen composite
Collagen gels were seeded with rabbit bone marrow-derived mesenchymal stem cells (MSCs) and contracted onto sutures at initial cell densities of 1, 4, and 8 million cells/ml. These MSC–collagen composites were then implanted into full thickness, full length, central defects created in the patellar t...
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Published in | Journal of orthopaedic research Vol. 21; no. 3; pp. 420 - 431 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Elsevier Ltd
01.05.2003
Wiley Subscription Services, Inc., A Wiley Company Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Collagen gels were seeded with rabbit bone marrow-derived mesenchymal stem cells (MSCs) and contracted onto sutures at initial cell densities of 1, 4, and 8 million cells/ml. These MSC–collagen composites were then implanted into full thickness, full length, central defects created in the patellar tendons of the animals providing the cells. These autologous repairs were compared to natural repair of identical defects on the contralateral side. Biomechanical, histological, and morphometric analyses were performed on both repair tissue types at 6, 12, and 26 weeks after surgery. Repair tissues containing the MSC–collagen composites showed significantly higher maximum stresses and moduli than natural repair tissues at 12 and 26 weeks postsurgery. However, no significant differences were observed in any dimensional or mechanical properties of the repair tissues across seeding densities at each evaluation time. By 26 weeks, the repairs grafted with MSC–collagen composites were one-fourth of the maximum stress of the normal central portion of the patellar tendon with bone ends. The modulus and maximum stress of the repair tissues grafted with MSC–collagen composites increased at significantly faster rates than did natural repairs over time. Unexpectedly, 28% of the MSC–collagen grafted tendons formed bone in the regenerating repair site. Except for increased repair tissue volume, no significant differences in cellular organization or histological appearance were observed between the natural repairs and MSC–collagen grafted repairs. Overall, these results show that surgically implanting tissue engineered MSC–collagen composites significantly improves the biomechanical properties of tendon repair tissues, although greater MSC concentrations produced no additional significant histological or biomechanical improvement. |
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Bibliography: | ark:/67375/WNG-XJ62WTR9-X istex:EB2DE52B928169AB9209C1875EC8757E1175998C ArticleID:JOR1100210308 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0736-0266 1554-527X |
DOI: | 10.1016/S0736-0266(02)00163-8 |